Related Experiment Video
Updated: Feb 15, 2026
07:18
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
Published on: May 12, 2023
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Nanoparticle effect on neutrophil produced myeloperoxidase
Elodie Sanfins1,2,3, Alexandra Correia4,5, Stefan B Gunnarsson1,2
1Biochemistry and Structural Biology, Lund University, Lund, Sweden.
Plos One
|January 19, 2018
Summary
Nanoparticles influence immune responses by affecting not just cells, but also released enzymes like myeloperoxidase. Their impact on enzyme activity depends on concentration and protein presence, revealing complex nanoparticle-immune interactions.
Area of Science:
- Immunology
- Nanotechnology
- Biochemistry
Background:
- Nanoparticles can interact with immune cells, potentially modulating immune responses.
- The interaction of nanoparticles with soluble immune factors, such as cytokines and enzymes, remains less understood.
Purpose of the Study:
- To investigate the effect of nanoparticles on the activity of myeloperoxidase, an enzyme released by activated neutrophils.
- To determine if nanoparticle chemistry and size influence their interaction with myeloperoxidase.
Main Methods:
- Measurement of myeloperoxidase activity in the presence of nanoparticles of varying chemistry and size.
- Assessment of the influence of protein concentration (bovine serum albumin) on nanoparticle-enzyme interactions.
Main Results:
- Nanoparticle concentration-dependently altered myeloperoxidase activity: high concentrations decreased activity, while low concentrations increased it.
- The presence of low concentrations of bovine serum albumin enhanced the effect of nanoparticles on myeloperoxidase activity.
- Nanoparticle effects were linked to the interplay between enzyme, protein concentrations, and nanoparticle surface area.
Conclusions:
- Nanoparticles modulate immune responses at both cellular and soluble effector levels.
- The activity of released immune effectors, like myeloperoxidase, is significantly influenced by nanoparticle characteristics and the surrounding biochemical environment.
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