Effect of dys-1 mutation on gene expression profile in space-flown Caenorhabditis elegans

Dystrophin-like dys-1 gene expression increases in the body wall muscles of Caenorhabditis elegans after spaceflight (SF). Here we used a dys-1(cx18) mutant to analyze the molecular adaptive responses of C. elegans to SF.

DNA microarrays were performed to identify differentially expressed genes between wild-type (WT) and dys-1 mutant worms after SF. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses, predicted human diseases, and screened out key genes for human muscle diseases with NextBio.

Gene expression was less affected by SF in the dys-1 mutant than in the WT worms. The dys-1 mutation influenced neuromuscular gene expression (neuropeptide genes, muscle-related genes, and dystrophin-related genes) under SF conditions, among which 15 genes were specifically regulated by dys-1. NextBio analysis predicted that cdka-1, lev-11, unc-27, and unc-94 genes might play critical roles in muscle atrophy.

dys-1 Potentially regulates the neuromuscular system in space. Muscle Nerve, 2018.