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Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
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Diffusing-wave spectroscopy in a standard dynamic light scattering setup.

Zahra Fahimi1,2, Frank J Aangenendt1,2,3, Panayiotis Voudouris1,2

  • 1Department of Mechanical Engineering, Eindhoven University of Technology, 5600MB Eindhoven, The Netherlands.

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|January 20, 2018
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Summary
This summary is machine-generated.

Diffusing-wave spectroscopy (DWS) can now be used with cylindrical cells, enabling microrheology in standard setups. Angle-dependent measurements provide a wider range of scales and a consistency check for scattering samples.

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Area of Science:

  • Physics
  • Materials Science
  • Biophysics

Background:

  • Diffusing-wave spectroscopy (DWS) analyzes dynamics in turbid media using photon diffusion models.
  • Standard DWS analysis requires known photon path length distributions, typically limited to flat sample cells.
  • Cylindrical sample cells, common in dynamic light scattering, lack analytical path length distributions for DWS.

Purpose of the Study:

  • To adapt Diffusing-wave spectroscopy (DWS) for use with cylindrical sample cells.
  • To enable DWS-based microrheology in standard dynamic light scattering instruments.
  • To develop a method for determining photon path length distributions in cylindrical geometries.

Main Methods:

  • Random-walk simulations to predict path length distributions in cylindrical cells.
  • Numerical calculation of path length distributions based on transport mean free path and detection angle.
  • Experimental validation using DWS microrheology on particle suspensions and gelatin.

Main Results:

  • Numerical path length distributions were obtained for cylindrical cells as a function of detection angle.
  • DWS microrheology measurements in cylindrical cells yielded accurate mean-square displacements and viscoelastic properties.
  • Angle-dependent measurements allowed variation of probed length/time scales and served as a consistency check.

Conclusions:

  • DWS microrheology is feasible in standard dynamic light scattering setups using cylindrical cells.
  • The developed method provides accurate material dynamics and expands accessible length and time scales.
  • Angle-dependent DWS analysis in cylindrical cells offers a robust approach for material characterization.