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Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...

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Updated: Feb 15, 2026

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Replication timing kept in LINE.

Rachel J O'Neill^1,2, Michael J O'Neill^3,2

¹Institute for Systems Genomics, University of Connecticut, Storrs, CT Rachel.oneill@uconn.edu.

The Journal of Cell Biology

|January 20, 2018

Summary

This summary is machine-generated.

Accurate chromosome replication timing between homologous chromosomes is crucial for stability. Researchers discovered that antisense LINE-1 transcripts in long noncoding RNAs maintain this synchronous, chromosome-wide replication timing.

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Area of Science:

Cell Biology
Genetics
Molecular Biology

Background:

Synchronous replication timing between homologous chromosomes is vital for genomic stability.
The mechanisms regulating this precise timing have not been fully understood.

Purpose of the Study:

To identify the molecular factors responsible for maintaining synchronous chromosome-wide replication timing between homologous chromosomes.

Main Methods:

Analysis of long noncoding RNAs (lncRNAs).
Identification and characterization of LINE-1 (L1) transcripts within lncRNAs.
Investigating the role of L1 transcripts in regulating replication timing.

Main Results:

Antisense LINE-1 transcripts within lncRNAs were identified as critical regulators.
These L1 transcripts play a key role in ensuring synchronous replication timing across the genome.
This finding provides a novel mechanism for maintaining chromosome stability.

Conclusions:

Antisense LINE-1 transcripts are essential for coordinating replication timing between homologous chromosomes.
This discovery sheds light on a previously unknown mechanism underlying chromosome stability.
The findings open new avenues for research into genome integrity and replication dynamics.