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Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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Cis-regulatory Sequences02:02

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
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Sequences01:29

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Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Draft Genome Sequence of Mycobacterium setense CSUR47.

Amar Bouam1, Anthony Levasseur1, Michel Drancourt2

  • 1Faculté de Médecine, Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, INSERM 1095, Marseille, France.

Genome Announcements
|January 20, 2018
PubMed
Summary

We sequenced the complete genome of Mycobacterium setense CSUR47, a rapidly growing mycobacterium. This provides a valuable resource for understanding mycobacterial genetics and developing new diagnostics or treatments.

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Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Mycobacterium setense is a rapidly growing mycobacterium species.
  • The CSUR47 strain was isolated from a human maxillary sinus infection.
  • Genomic data for M. setense is limited.

Purpose of the Study:

  • To report the complete genome sequence of Mycobacterium setense CSUR47.
  • To provide a genomic resource for M. setense research.
  • To facilitate comparative genomics within the Mycobacterium genus.

Main Methods:

  • Whole-genome sequencing of M. setense CSUR47.
  • Bioinformatic analysis of the resulting genome sequence.
  • Annotation of protein-coding genes, tRNAs, and rRNAs.

Main Results:

  • The complete genome sequence of M. setense CSUR47 was determined to be 6,278,097 bp.
  • The genome exhibits a GC content of 66.40%.
  • The genome encodes 5,863 protein-coding genes, 48 tRNAs, and 9 rRNAs.

Conclusions:

  • The genome sequence of M. setense CSUR47 is now available.
  • This genomic data will aid in future research on M. setense.
  • Further studies can utilize this data for understanding mycobacterial pathogenesis and evolution.