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Related Experiment Videos

Decrease of the plasma membrane H+-ATPase activity during late exponential growth of Saccharomyces cerevisiae.

P Tuduri, E Nso, J P Dufour

    Biochemical and Biophysical Research Communications
    |December 31, 1985
    PubMed
    Summary

    Yeast plasma membrane H+-ATPase activity significantly decreases during the final cell division of exponential growth. This arrest growth control mechanism does not alter vanadate sensitivity, suggesting a specific regulatory pathway.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • The plasma membrane H+-ATPase (proton pump) is crucial for maintaining cellular pH and membrane potential in yeast.
    • Cellular growth and division are tightly regulated processes involving complex molecular mechanisms.

    Purpose of the Study:

    • To investigate the regulation of yeast plasma membrane H+-ATPase activity during the transition from exponential growth to cell cycle arrest.
    • To determine if changes in H+-ATPase activity during growth arrest correlate with altered sensitivity to vanadate.

    Main Methods:

    • Enzyme activity assays measuring H+-ATPase function in yeast plasma membrane preparations.
    • Analysis of vanadate inhibition kinetics to assess changes in enzyme sensitivity.

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    Main Results:

    • A significant two- to three-fold decrease in H+-ATPase activity was observed during the last cell division preceding growth arrest.
    • The observed reduction in ATPase activity was independent of changes in vanadate sensitivity.

    Conclusions:

    • Yeast employs a specific "arrest growth control" mechanism to downregulate plasma membrane H+-ATPase activity at the end of exponential growth.
    • This regulation appears to target the enzyme's activity directly rather than altering its interaction with vanadate.