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Partial characterization of synovial fluid nucleotide pyrophosphohydrolase
Arthritis and Rheumatism
|December 1, 1985
Summary
Synovial fluid adenosine triphosphate pyrophosphohydrolase activity increases in joint diseases like osteoarthritis. This enzyme is a heat-labile, cation-dependent pyrophosphohydrolase with optimal activity at alkaline pH.
Area of Science:
- Biochemistry
- Enzymology
- Rheumatology
Background:
- Synovial fluid adenosine triphosphate pyrophosphohydrolase activity is elevated in chondrocalcinosis and osteoarthritis.
- Understanding this enzyme's properties is crucial for investigating arthropathies.
Purpose of the Study:
- To partially characterize the enzyme adenosine triphosphate pyrophosphohydrolase in synovial fluid.
- To investigate the enzyme's properties in patients with various joint diseases.
Main Methods:
- Analysis of synovial fluid samples from 41 patients with diverse arthropathies.
- Enzyme activity assays to determine biochemical properties.
Main Results:
- The enzyme is soluble and heat-labile.
- It demonstrates divalent cation dependency and acts as a nonspecific nucleotide pyrophosphohydrolase.
- The optimal pH for activity was determined to be 9.0-9.5.
Conclusions:
- Synovial fluid adenosine triphosphate pyrophosphohydrolase is a characterized enzyme with specific properties.
- Its increased activity in certain arthropathies warrants further investigation into its role in joint disease pathogenesis.