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Updated: Feb 15, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Comprehensive analysis of glycosaminoglycans from the edible shellfish
Yusuke Okamoto1, Kyohei Higashi1, Robert J Linhardt2
1Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
Abstract:
We have previously reported that the keratan sulfate (KS) disaccharide was branched to the C-3 position of glucuronate in chondroitin sulfate (CS)-E derived from the Mactra chinensis. We carried out the comprehensive disaccharide analysis of GAGs from 10 shellfish, Ruditapes philippinarum, Scapharca broughtonii, Mizuhopecten yessoensis, Turbo cornutus, Crassostrea nippona, Corbicula japonica, Mytilus galloprovincialis, Neptunea intersculpta, Pseudocardium sachalinense and Crassostrea gigas, to better understand the glycan structures in marine organisms. The contents of CS, heparan sulfate and hyaluronic acid and their compositions depend on the species of shellfish. Interestingly, a peak corresponding to a pentasaccharide containing KS disaccharide was observed when GAGs from T. cornutus was treated with chondroitinase (Chase) ACII but not Chase ABC. In addition, unidentified peaks were also observed when CS derived from R. philippinarum, S. broughtonii were treated with Chase ACII. These results suggest the presence of additional unidentified structure of CS in these shellfish.
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