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Updated: Feb 15, 2026

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
Published on: July 10, 2015
Induction by innate defence regulator peptide 1018 of pro-angiogenic molecules and endothelial cell migration in a
Paulina Marin-Luevano1, Valentin Trujillo1, Adrian Rodriguez-Carlos2
1Medical Research Unit-Zacatecas, Mexican Institute for Social Security-IMSS, Zacatecas, Mexico; Department of Immunology, School of Medicine, Universidad Autonoma de San Luis Potosi, Mexico.
Abstract:
Synthetic innate defence regulator (IDR) peptides such as IDR-1018 modulate immunity to promote key protective functions including chemotaxis, wound healing, and anti-infective activity, while suppressing pro-inflammatory responses to non-pathological levels. Here we demonstrated that IDR-1018 induced, by up to 75-fold, pro-angiogenic VEGF-165 in keratinocytes but suppressed this isoform in endothelial cells. It also induced early angiogenin and prolonged anti-inflammatory TGFβ expression on endothelial cells, while suppressing early pro-inflammatory IL-1β expression levels. IDR-1018 also down-regulated the hypoxia induced transcription factor HIF-1α in both keratinocytes and endothelial cells. Consistent with these data, in an in vitro wound healing scratch assay, IDR-1018 induced migration of endothelial cells under conditions of hypoxia while in epithelial cells migration increased only under conditions of normoxia.
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