Related Experiment Video
Updated: Feb 15, 2026
11:41
Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
Published on: December 16, 2018
6.1K
Functionally compromised synovium-derived mesenchymal stem cells in Charcot neuroarthropathy.
Reed Mitchell1, Jeremy Molligan2, Sydney Rooney3
1Orthobiologic Laboratory, MedStar Union Memorial Hospital, Baltimore, MD, United States.
Experimental and Molecular Pathology
|January 22, 2018
Summary
Synovial mesenchymal stem cells (syn-MSCs) are fewer and less potent in Charcot neuroarthropathy (CNA) patients. These stem cells show reduced differentiation capacity, impacting CNA pathogenesis.
Area of Science:
- Orthopedics and Regenerative Medicine
- Diabetic Foot Complications Research
Background:
- Charcot neuroarthropathy (CNA) is a severe diabetic foot complication.
- The specific role of synovial mesenchymal stem cells (syn-MSCs) in CNA pathogenesis remains largely unknown.
Purpose of the Study:
- To comparatively analyze syn-MSCs from diabetic patients with CNA versus non-diabetic controls.
- To investigate the quantity, colony formation, and differentiation potential of syn-MSCs in CNA joints.
Main Methods:
- Synovial samples were collected from diabetic patients with CNA (n=7) and non-diabetic controls (n=7).
- Syn-MSCs were isolated and characterized for colony formation, size, density, and differentiation potential (adipogenic, osteogenic, chondrogenic).
- Gene expression (PPAR-γ, RUNX2, Sox9, type II collagen) was assessed during differentiation.
Main Results:
- Significantly fewer syn-MSCs and reduced colony formation were observed in the CNA group compared to controls.
- Syn-MSCs from CNA patients exhibited smaller colony sizes and decreased density in high-density subgroups.
- Reduced expression of key differentiation markers (PPAR-γ, RUNX2, Sox9, type II collagen) was noted in CNA syn-MSCs.
Conclusions:
- Syn-MSCs in CNA joints are reduced in number and possess diminished differentiation capabilities.
- The high-density subpopulation of syn-MSCs is particularly compromised in the context of Charcot neuroarthropathy.
- These findings suggest impaired stem cell function contributes to the pathogenesis of diabetic Charcot neuroarthropathy.
More Related Videos
10:50Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
4.3K
04:47Author Spotlight: Advancements in Stem Cell-Derived Vascularized Organoids and Optimized Isolation of Wharton's Jelly for Enhanced MSC Production
Published on: July 5, 2024
4.3K
Related Concept Videos
01:19Mesenchymal Stem Cells
5.7K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.7K
01:26Derivatives of the Trigonometric Functions
500
The motion of a Ferris wheel rotating at a constant speed provides an intuitive model for understanding trigonometric functions and their derivatives. As a rider moves along the circular path, the vertical height above the ground changes smoothly and periodically over time. This vertical motion can be accurately represented by a sine function, reflecting the repeating pattern of ascent and descent inherent to circular motion.Height and Rate of ChangeIf the rider’s height is modeled by a...
500
01:22Derivatives of Logarithmic Functions
110
Logarithmic and Exponential RelationshipA logarithmic function is the inverse of an exponential function. If y = logb x then, it can be rewritten as by = x. This relationship allows for implicit differentiation, making logarithmic functions useful in calculus. Logarithmic scales are widely used to represent data that span multiple orders of magnitude, such as earthquake magnitudes (Richter scale) and sound intensity (decibels).Differentiation of Logarithmic FunctionsTo differentiate y = logb x,...
110
01:33Adult Stem Cells
33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
01:29Second Derivatives of Implicit Functions
89
Elliptical arches are fundamental in architectural and structural engineering, offering aesthetic appeal and structural efficiency. The shape of an elliptical arch follows a constrained geometric relationship where the height and horizontal position are implicitly related. This means that the height y cannot be explicitly expressed as a function of the horizontal position x, necessitating implicit differentiation for slope and curvature analysis.The equation of an ellipse centered at the origin...
89
01:27Derivatives of Simple Functions
176
Derivatives quantify the rate of change of a function and can be interpreted geometrically as the slope of a straight line or the slope of a tangent line to a curve at a given point. In the context of a roller coaster, the derivative of the function describing the track’s horizontal position provides a mathematical description of how steep the path is at any location along the ride.Constant and Linear PathsA horizontal segment of a roller coaster can be modeled by a constant function,...
176