Related Experiment Video

Updated: Feb 15, 2026

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
10:19

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

Published on: June 26, 2014

13.7K

SesI May Be Associated with the Invasiveness of Staphylococcus epidermidis

Xiuqin Qi1, Ye Jin1, Jingjing Duan1

  • 1Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Frontiers in Microbiology
|January 23, 2018
PubMed

Insights

Staphylococcus epidermidis surface protein I (SesI) promotes bacterial adhesion and aggregation, particularly in the ST2 clone. This virulence factor is linked to increased antimicrobial resistance and biofilm gene prevalence in invasive strains.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Staphylococcus epidermidis is a common skin bacterium that can cause infections.
  • The role of its surface protein I (SesI) in pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the prevalence and role of the sesI gene in S. epidermidis virulence.
  • To determine the association of sesI with antimicrobial resistance and biofilm formation.

Main Methods:

  • Prevalence study of sesI in invasive and colonizing S. epidermidis isolates.
  • Analysis of biofilm-associated and antimicrobial resistance genes.
  • Antimicrobial susceptibility testing.
  • Multilocus sequence typing (MLST) to identify sequence types (STs).
  • Construction of sesI gene mutant and complementary strains.

Main Results:

  • The sesI gene was significantly more prevalent in invasive (20.8%) than colonizing (3.8%) S. epidermidis isolates.
  • SesI-positive isolates showed higher rates of biofilm-associated genes (aap, icaA, IS256) and the mupA resistance gene.
  • SesI-positive isolates exhibited increased resistance to ciprofloxacin, gentamicin, and trimethoprim/sulfamethoxazole.
  • A strong association was found between sesI carriage and the ST2 clone (80.8% of sesI-positive isolates).
  • Experimental data suggested sesI promotes S. epidermidis adhesion and aggregation but not mature biofilm formation.

Conclusions:

  • The sesI gene is associated with increased virulence, antimicrobial resistance, and specific clones (ST2) of S. epidermidis.
  • SesI likely contributes to bacterial colonization and adhesion, facilitating the spread of S. epidermidis infections.
Keywords:
Staphylococcus epidermidisadhesionaggregationbiofilmsesIvirulence-associated genes

More Related Videos

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus10:39

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Published on: March 10, 2017

17.4K
Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS08:54

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS

Published on: June 8, 2011

18.6K

Related Concept Videos

Mechanical Ventilation II: Invasive Ventilation01:23

Mechanical Ventilation II: Invasive Ventilation

Ventilators are essential medical equipment used to aid patients with respiratory difficulties. Their primary function is to assist or replace spontaneous breathing by providing mechanical ventilation. There are two general classes of mechanical ventilators: negative-pressure and positive-pressure ventilators.
Negative-Pressure Ventilators
Negative-pressure ventilators create a vacuum around the chest or body to draw air into the lungs, simulating breathing. This method does not require an...
805
Threats to Biodiversity01:50

Threats to Biodiversity

There have been five major extinction events throughout geological history, resulting in the elimination of biodiversity, followed by a rebound of species that adapted to the new conditions. In the current geological epoch, the Holocene, there is a sixth extinction event in progress. This mass extinction has been attributed to human activities and is thus provisionally called the Anthropocene. In 2019 the human population reached 7.7 billion people and is projected to comprise 10 billion by...
27.2K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
63.7K