Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.9K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.9K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

32.9K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.9K
RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
RNA Splicing01:32

RNA Splicing

60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
Support Reactions in Three Dimensions01:27

Support Reactions in Three Dimensions

1.7K
Support reactions in three dimensions help maintain the stability and equilibrium of various structures and systems. These reactions prevent the system from translating and rotating, ensuring the design can withstand external forces and perform its intended function efficiently and safely. Some of the supports providing support reactions in three dimensions are discussed below:
Ball and Socket Joint is one of the supports allowing free rotation about any axis. This freedom of rotation is...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential susceptibility of hair colour changes after barzolvolimab.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same author

Histone H4 acetyl-methyllysine marks accessible chromatin that resists compaction.

bioRxiv : the preprint server for biology·2026
Same author

Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation.

Nucleic acids research·2026
Same author

Performance of omalizumab in patients with mast cell-mediated angioedema with and without wheals.

The journal of allergy and clinical immunology. In practice·2026
Same author

The role of cohesin and DNA loop extrusion in the generation of single neuron identity.

Current opinion in genetics & development·2026
Same author

Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation.

bioRxiv : the preprint server for biology·2025
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

11.3K

TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding.

Jeremy A Schofield1,2, Erin E Duffy1,2, Lea Kiefer1,2

  • 1Department of Molecular Biophysics & Biochemistry, Yale University, New Haven, Connecticut, USA.

Nature Methods
|January 23, 2018
PubMed
Summary
This summary is machine-generated.

TimeLapse-seq introduces a novel method to track RNA dynamics. This technique uses chemical modifications to mark newly synthesized RNA, revealing temporal information lost in standard RNA sequencing.

More Related Videos

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

9.6K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.3K

Related Experiment Videos

Last Updated: Feb 15, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

11.3K
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

9.6K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.3K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Traditional RNA sequencing (RNA-seq) provides a static view of RNA populations.
  • Current methods lack the ability to capture temporal dynamics of RNA synthesis and degradation.

Purpose of the Study:

  • To develop a novel RNA sequencing technique that captures temporal information.
  • To enable the study of RNA dynamics and induced differential expression.

Main Methods:

  • TimeLapse-seq utilizes oxidative-nucleophilic-aromatic substitution.
  • 4-thiouridine is converted into cytidine analogs, creating U-to-C mutations.
  • This method marks newly synthesized transcripts for sequencing.

Main Results:

  • TimeLapse-seq successfully marks new transcripts with apparent U-to-C mutations.
  • The single-molecule approach reveals RNA dynamics previously hidden in traditional RNA-seq.
  • Induced differential expression can be accurately assessed over time.

Conclusions:

  • TimeLapse-seq is a versatile and adaptable single-molecule technique.
  • This method overcomes the limitations of traditional RNA-seq for studying RNA dynamics.
  • TimeLapse-seq opens new avenues for understanding RNA biology and gene regulation.