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Updated: Feb 15, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
Published on: July 10, 2019
TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding
Jeremy A Schofield1,2, Erin E Duffy1,2, Lea Kiefer1,2
1Department of Molecular Biophysics & Biochemistry, Yale University, New Haven, Connecticut, USA.
Abstract:
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.
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