Related Experiment Video

Updated: Feb 15, 2026

Porcine As a Training Module for Head and Neck Microvascular Reconstruction
07:43

Porcine As a Training Module for Head and Neck Microvascular Reconstruction

Published on: September 29, 2018

8.2K

Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis

Chung-Hsien Chou1, Hsi-Feng Tu2, Shou-Yen Kao2,3

  • 1Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.

Head & Neck
|January 23, 2018
PubMed
Abstract

Insights

MicroRNAs miR-31, miR-96, and miR-182 are upregulated in head and neck squamous cell carcinoma (HNSCC). These miRNAs co-target Numb, influencing cancer cell migration and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are critical regulators in head and neck squamous cell carcinoma (HNSCC).
  • The specific roles of miR-31, miR-96, and miR-182 in HNSCC pathogenesis require elucidation.
  • Investigating the targeting of Numb by these miRNAs is essential for understanding HNSCC progression.

Purpose of the Study:

  • To determine the expression levels of miR-31, miR-96, and miR-182 in HNSCC tissues.
  • To investigate the direct targeting of the Numb gene by miR-31, miR-96, and miR-182.
  • To analyze the functional impact of these miRNAs on HNSCC cell behavior.

Main Methods:

  • Analysis of miR-31, miR-96, and miR-182 expression in HNSCC tumor tissues.
  • Utilizing reporter assays and knockdown experiments in HNSCC cell lines.
  • Performing oncogenic and expression analyses to assess miRNA function.

Main Results:

  • Concordant upregulation of miR-31, miR-96, and miR-182 was observed in HNSCC tumor tissues, correlating with advanced tumor stages.
  • These miRNAs were found to directly target the 3' untranslated region (UTR) of the Numb gene.
  • Exogenous expression of miR-31/96/182 enhanced HNSCC cell migration and invasion, effects reversed by Numb expression.

Conclusions:

  • miR-31, miR-96, and miR-182 are frequently and coordinately upregulated in HNSCC.
  • These miRNAs act as oncogenes by co-targeting Numb, thereby promoting HNSCC progression.
  • The findings highlight a novel miRNA-Numb axis in HNSCC development.
Keywords:
NumbcarcinomamiR-182miR-31miR-96mouth

More Related Videos

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma08:59

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Published on: January 5, 2017

11.4K
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

28.3K

Related Concept Videos

Arteries of the Head and Neck01:26

Arteries of the Head and Neck

The human body's intricate network of arteries ensures that every organ system receives the necessary oxygen and nutrients for optimal function. The arterial network in the head and neck region is particularly complex, providing vital blood flow to the brain, eyes, and other critical structures. Prominent arteries in this region include the internal carotid arteries and the vertebral arteries.
The internal carotid arteries supply blood to the anterior portion of the cerebrum. They enter the...
3.7K
Veins of Head and Neck01:19

Veins of Head and Neck

The blood drainage from the head and neck is primarily managed by three pairs of veins: the external jugular, internal jugular, and vertebral veins. The external jugular veins drain superficial scalp and face structures, passing over the sternocleidomastoid muscles to empty into the subclavian veins.
On the other hand, the vertebral veins, unlike their arterial counterparts, are not primarily responsible for brain drainage. Instead, they drain the cervical vertebrae, spinal cord, and some small...
5.9K
Other Nuclides: 31P, 19F, 15N NMR01:16

Other Nuclides: 31P, 19F, 15N NMR

Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research.
While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a...
806
Muscles of the Anterior Neck01:26

Muscles of the Anterior Neck

The anterior neck muscles are the group of muscles covering the front part of the neck. These muscles are classified into three subgroups. The first one is the superficial muscles, the most visible muscles in the front of the neck. It includes the platysma and sternocleidomastoid. The second group is the suprahyoid muscles, located above the hyoid bone. This group comprises the digastric, mylohyoid, geniohyoid, and stylohyoid. Lastly, the infrahyoid muscles are found below the hyoid bone and...
4.9K
Muscles that Move the Head01:19

Muscles that Move the Head

The muscles that move the head are a dynamic and complex group of structures that work together to facilitate a wide range of head movements, including rotation, flexion, extension, and lateral bending.
The bilateral sternocleidomastoid, or SCM, and the suprahyoid and infrahyoid muscles are significant head flexors. The SCM muscles originate at the sternum and clavicle and attach to the mastoid process of the temporal bone. The SCM contracts bilaterally to bend the head forward, whereas...
6.2K
Gene Flow02:39

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
38.1K