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Related Concept Videos

Transcription01:10

Transcription

157.3K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.3K
Hybrid Zones02:29

Hybrid Zones

22.0K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
22.0K
Transcription Factors02:16

Transcription Factors

82.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.9K
Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

68.0K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
68.0K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.1K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.9K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.9K

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Related Experiment Video

Updated: Feb 15, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
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In Situ Hybridization for the Precise Localization of Transcripts in Plants

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Hybridization Histochemistry of Neural Transcripts.

W Scott Young1, June Song1, Éva Mezey2

  • 1Section on Neural Gene Expression, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland.

Current Protocols in Neuroscience
|January 23, 2018
PubMed
Summary

This unit details methods for locating RNA transcripts in tissues using various probes, including modified oligodeoxynucleotides and ribonucleic acids. Optimized probe sets offer high sensitivity and specificity for accurate RNA detection.

Keywords:
ViewRNAdigoxigeninin situ hybridizationmRNAriboprobes

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Accurate localization of RNA transcripts within tissues is crucial for understanding gene expression and cellular function.
  • Existing methods for RNA detection may vary in sensitivity, specificity, and applicability.

Purpose of the Study:

  • To present a comprehensive unit detailing protocols for the in situ detection of RNA transcripts in tissues.
  • To describe various probe types and hybridization strategies for enhanced RNA localization.

Main Methods:

  • Utilizing radiolabeled or colorimetric probes for RNA transcript detection.
  • Employing modified oligodeoxynucleotides (single or paired) and ribonucleic acids as probes.
  • Implementing hybridization techniques for specific probe-target binding within tissue sections.

Main Results:

  • Achieving high sensitivity and specificity in RNA transcript localization.
  • Demonstrating the effectiveness of paired oligodeoxynucleotide probes for superior detection.
  • Validating the utility of diverse probe types for different experimental needs.

Conclusions:

  • The presented protocols provide robust methods for sensitive and specific RNA transcript localization in diverse tissue types.
  • Modified oligodeoxynucleotide pairs represent a highly effective strategy for advanced RNA detection in molecular biology research.