Related Experiment Video
Updated: Feb 15, 2026
14:49
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Published on: October 27, 2011
39.7K
nextPARS: parallel probing of RNA structures in Illumina
Ester Saus1,2, Jesse R Willis1,2, Leszek P Pryszcz1,2,3
1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona 08003, Spain.
RNA (New York, N.Y.)
|January 24, 2018
Summary
We developed nextPARS, a new method for RNA structure probing. This technique offers high accuracy and increased throughput for analyzing RNA molecules in biological samples.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- RNA molecules are crucial for cellular processes, with their functions dependent on specific structures.
- Understanding RNA structure is key to deciphering RNA function, interactions, and evolution.
- Recent advancements include sequencing-based methods for large-scale RNA structure analysis.
Purpose of the Study:
- To introduce nextPARS, a novel method for in vitro parallel probing of RNA structures.
- To provide a high-throughput, multiplexing-capable Illumina-based approach for RNA structure determination.
Main Methods:
- nextPARS utilizes Illumina sequencing for in vitro parallel probing of RNA structures.
- The method is designed for high-throughput analysis of RNA structural features.
- Sample multiplexing is incorporated to increase efficiency.
Main Results:
- nextPARS demonstrates accuracy comparable to existing RNA structure probing techniques.
- The method significantly enhances throughput for RNA structure analysis.
- nextPARS enables simultaneous analysis of multiple samples.
Conclusions:
- nextPARS is a powerful new tool for RNA structure determination.
- The approach offers advantages in terms of speed and capacity for RNA research.
- This method facilitates a deeper understanding of RNA structure-function relationships.
Related Concept Videos
01:23RNA Structure
79.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.3K
01:19RNA Structure
7.8K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.8K
02:41Chromatin Structure and RNA Splicing
3.5K
3.5K
01:20Parallel Processing
780
The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
780
01:23Parallel Resonance
614
The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
614
01:53RNA Stability
35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K