Related Experiment Video

Updated: Feb 15, 2026

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
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Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Published on: December 5, 2020

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Targeting cleavage and polyadenylation specific factor 1 via shRNA inhibits cell proliferation in human ovarian

Beiguang Zhang1, Ying Liu, Donghui Liu

  • 1Department of Intervention Therapy, Jilin Cancer Hospital, Changchun, Jilin, China.

Journal of Biosciences
|January 24, 2018
PubMed

Insights

Cleavage and polyadenylation specificity factor 1 (CPSF1) is overexpressed in ovarian cancer. Depleting CPSF1 inhibits cancer cell growth, proliferation, and survival, suggesting it

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Cleavage and polyadenylation specificity factor 1 (CPSF1) is crucial for pre-mRNA 3'-end formation.
  • The role of CPSF1 in ovarian cancer pathogenesis is not well understood.
  • Ovarian cancer remains a significant global health challenge requiring novel therapeutic targets.

Purpose of the Study:

  • To investigate the expression levels of CPSF1 in ovarian cancer tissues.
  • To elucidate the functional role of CPSF1 in ovarian cancer cell behavior.
  • To assess the potential of CPSF1 as a therapeutic target for ovarian cancer.

Main Methods:

  • Analysis of CPSF1 mRNA expression using the Oncomine Cancer Microarray database.
  • Loss-of-function studies utilizing CCK-8, colony formation, and flow cytometry assays.
  • Assessment of apoptosis-related proteins (cleaved caspase-3, PARP) and cell cycle regulators (CDK4/cyclin D1) following CPSF1 knockdown.

Main Results:

  • CPSF1 mRNA was significantly overexpressed in ovarian cancer tissues compared to normal tissues.
  • CPSF1 depletion led to reduced cell viability, impaired colony formation, G0/G1 cell cycle arrest, and increased apoptosis in ovarian cancer cell lines (SKOV-3, OVCAR-3).
  • Knockdown of CPSF1 resulted in elevated cleaved caspase-3 and PARP, and decreased CDK4/cyclin D1 expression.

Conclusions:

  • CPSF1 promotes ovarian cancer cell growth and proliferation in vitro.
  • The findings suggest CPSF1 plays a oncogenic role in ovarian cancer.
  • CPSF1 represents a potential therapeutic target for human ovarian cancer treatment.

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