Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lagging Strand Synthesis01:59

Lagging Strand Synthesis

61.6K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
61.6K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

16.9K
16.9K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.8K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.8K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

15.0K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
15.0K
DNA Replication02:40

DNA Replication

60.5K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
60.5K
The DNA Replication Fork01:02

The DNA Replication Fork

41.2K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temporal Trends in Cancer Burden and 27 Subtypes Among Women of Childbearing Age Globally from 1990 to 2021.

International journal of women's health·2026
Same author

Benmelstobart Plus Anlotinib Is Unlikely to Be Cost-Effective for Advanced Renal Cell Carcinoma: An Integrated Disease Burden and Cost-Effectiveness Analysis.

Inquiry : a journal of medical care organization, provision and financing·2026
Same author

Temporal Trends in Mortality of Female Cancers Among Women and the Impact of COVID-19 in Low- and Middle-Income Countries, 1990-2023: An Age-Period-Cohort Analysis from the Global Burden of Disease Study 2023.

International journal of women's health·2026
Same author

Global burden, trends, and inequalities in cancer and subtypes attributable to high BMI among older adults, 1990-2021: a secondary analysis of the global burden of disease study 2021.

Frontiers in nutrition·2025
Same author

Global burden, health inequalities and improvement gap of head and neck cancers in middle-aged and older adults from 1990 to 2021.

PloS one·2025
Same author

Time-space analysis of gastrointestinal cancers among the working-age individuals in Brazil, Russia, India, China, and South Africa (BRICS)-plus countries (1990-2021): a comprehensive analysis from the Global Burden of Disease Study 2021.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2025

Related Experiment Video

Updated: Feb 15, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K

Programmable autonomous synthesis of single-stranded DNA.

Jocelyn Y Kishi1,2, Thomas E Schaus1,2, Nikhil Gopalkrishnan1,2

  • 1Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.

Nature Chemistry
|January 24, 2018
PubMed
Summary
This summary is machine-generated.

Primer Exchange Reaction (PER) cascades enable autonomous DNA synthesis for novel molecular devices. This technology allows for programmable, in situ DNA growth, advancing nanotechnology and biotechnology applications.

More Related Videos

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
13:46

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

32.4K
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

7.8K

Related Experiment Videos

Last Updated: Feb 15, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
13:46

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

32.4K
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

7.8K

Area of Science:

  • Molecular Biology
  • Nanotechnology
  • Biotechnology

Background:

  • Current methods for autonomous single-stranded DNA synthesis are limited.
  • DNA's diverse functional roles in biology, nanotechnology, and biotechnology necessitate improved synthesis techniques.

Purpose of the Study:

  • To introduce Primer Exchange Reaction (PER) cascades for autonomous DNA synthesis.
  • To provide a platform for engineering molecular circuits and devices with advanced capabilities.

Main Methods:

  • Development of Primer Exchange Reaction (PER) cascades for user-specified single-stranded DNA synthesis.
  • Programmable, autonomous, in situ, and environmentally responsive DNA synthesis.

Main Results:

  • Demonstration of a nanodevice transducing RNA detection into DNAzyme production.
  • Creation of a signal amplifier for conditional fluorescent strand synthesis based on RNA signals.
  • Implementation of molecular computing circuits for RNA input logic operations (AND, OR, NOT).
  • Development of a temporal molecular event recorder tracking sequential RNA input detection.

Conclusions:

  • PER cascades offer a versatile platform for creating sophisticated molecular devices.
  • The demonstrated applications highlight the potential for sensing, signal processing, and recording in molecular systems.