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Related Concept Videos

MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
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Related Experiment Video

Updated: Feb 15, 2026

Enucleation of the Prostate for the Treatment of Benign Prostatic Hyperplasia Using a 980 nm Diode Laser
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MicroRNA expression profiles in benign prostatic hyperplasia.

Nan Zhang1, Zhongyi Li1, Fuding Bai1

  • 1Department of Urology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.

Molecular Medicine Reports
|January 24, 2018
PubMed
Summary
This summary is machine-generated.

This study identified specific microRNAs (miRNAs) altered in benign prostate hyperplasia (BPH) stromal tissue. These findings offer insights into BPH molecular mechanisms and potential therapeutic targets.

Keywords:
microRNAbenign prostatic hyperplasiamicroRNA-96-5p

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Area of Science:

  • Urology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA (miRNA) expression changes are known in prostate cancer, but their role in benign prostate hyperplasia (BPH) stromal tissue is not fully understood.
  • Investigating miRNA expression in BPH is crucial for elucidating the molecular underpinnings of this common condition.

Purpose of the Study:

  • To investigate miRNA and gene expression profiles in benign prostate hyperplasia (BPH) stromal tissue.
  • To identify differentially expressed miRNAs and their potential target genes in BPH.
  • To establish a foundation for future functional studies on BPH regulatory mechanisms.

Main Methods:

  • Microarray analysis of miRNA and mRNA expression in BPH and healthy prostate tissue.
  • Validation of differentially expressed miRNAs using reverse transcription quantitative polymerase chain reaction (RT-qPCR).
  • Analysis of prostate tissue samples from 30 BPH patients and 5 healthy controls.

Main Results:

  • Eight miRNAs were found to be upregulated, and eight were downregulated in BPH tissue.
  • MicroRNA-96-5p was identified as having an inhibitory effect on the mRNA expression of several genes, including MTOR and caspase 2.
  • RT-qPCR analysis confirmed 16 differentially expressed miRNAs.

Conclusions:

  • This study identified specific differentially expressed miRNAs in the stromal tissue of benign prostate hyperplasia.
  • The findings suggest potential regulatory roles for these miRNAs in BPH pathogenesis.
  • Further functional studies are warranted to explore the precise mechanisms by which these miRNAs influence BPH.