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MicroRNA regulation of MDM2-p53 loop in pterygium

Yufei Teng1, Gary Hin-Fai Yam2, Na Li3

  • 1Department of Ophthalmology & Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China; Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China.

Experimental Eye Research
|January 24, 2018
PubMed
Abstract

Insights

MicroRNA-145 (miR-145) is elevated in pterygium, suppressing MDM2 and influencing p53. This miR-145/MDM2-p53 pathway presents a potential therapeutic target for pterygium treatment.

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Epigenetics

Background:

  • Pterygium pathogenesis involves limbal stem cell damage, abnormal apoptosis, and cellular proliferation.
  • Epigenetic regulation via microRNA expression is implicated in pterygium development.

Purpose of the Study:

  • To investigate the role of microRNA expression in pterygium pathogenesis.
  • To explore the epigenetic regulation of pterygium through microRNA analysis.

Main Methods:

  • MicroRNA and mRNA expression analyzed using quantitative polymerase chain reaction (qPCR).
  • MicroRNA tissue localization performed via in situ hybridization.
  • Functional effects of microRNA-145 (miR-145) on pterygial cells assessed through transfection, cell cycle analysis, apoptosis assays, and gene expression analysis (p53, MDM2).

Main Results:

  • miR-145 expression was significantly higher in pterygium tissue compared to limbus and conjunctiva.
  • miR-145 and miR-143 were predominantly expressed in the basal pterygial epithelium.
  • Ectopic miR-145 expression in pterygial cells induced cell cycle arrest (G1), downregulated MDM2, and upregulated p53.

Conclusions:

  • miR-145 suppresses MDM2 expression, impacting p53-mediated cell growth in pterygial epithelium.
  • The miR-145/MDM2-p53 regulatory loop is a potential therapeutic target for pterygium treatment.
Keywords:
MDM2-p53 loopPterygiummiR-145microRNA

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