Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Determination of interleukin 1].

H Friemel, M Nausch

    Allergie Und Immunologie
    |January 1, 1985
    PubMed
    Summary

    Interleukin 1 (IL-1) is crucial for T cell activation. This study details a method using mouse thymocytes to measure IL-1

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Immunohistochemical characterization of the pancreatic cellular infiltrate in normal pancreas, chronic pancreatitis and pancreatic carcinoma.

    Digestion·1998
    Same author

    In vitro effects of solubilized HLA-DR--role in immunoregulation?

    Cellular immunology·1994
    Same author

    The so-called interleukin-2 inhibitory activity of human serum is largely cytotoxic to mouse cells.

    Immunology letters·1990
    Same author

    Are soluble monocyte-derived HLA class II molecules candidates for immunosuppressive activity?

    Immunology letters·1990
    Same author

    [Interleukins 1-8].

    Allergie und Immunologie·1990
    Same author

    [Clinical value of interleukin 1- and interleukin 2-determinations in patients after kidney transplantation].

    Allergie und Immunologie·1990

    Area of Science:

    • Immunology
    • Cellular Biology

    Background:

    • Interleukin 1 (IL-1) plays a vital role in macrophage-dependent T cell activation.
    • Assessing IL-1's comitogenic activity with mouse thymocytes is a key method for its determination.
    • Standard IL-1 preparations involve stimulating macrophage cell lines (P388D1) or human leukocytes with lipopolysaccharide (LPS).

    Purpose of the Study:

    • To establish and validate a method for quantifying Interleukin 1 (IL-1) using its comitogenic activity.
    • To investigate the optimal conditions and factors influencing IL-1 determination in mouse thymocyte assays.

    Main Methods:

    • Utilizing mouse thymocytes from five different strains to assess mitogenic activity.
    • Stimulating macrophage cell line P388D1 or human leukocytes with LPS to generate IL-1 standards.
    • Evaluating thymocyte susceptibility to IL-1, noting its slower development compared to Concanavalin A (Con A) reactivity.
    • Ensuring animals used for thymocyte preparation are between 8 to 10 weeks old.
    • Accounting for potential interference from IL-1 and IL-2 inhibitors during IL-1 determination.

    Main Results:

    • Lipopolysaccharide (LPS) does not interfere with IL-1 determination within the tested concentration range (10^-3 to 10^2 µg/mL).
    • Thymocyte susceptibility to IL-1 develops gradually, differing from the faster Concanavalin A (Con A) response.
    • Optimal thymocyte preparation requires animals aged 8-10 weeks.

    Conclusions:

    • A reliable method for determining Interleukin 1 (IL-1) activity using mouse thymocytes is presented.
    • Careful consideration of thymocyte age and potential inhibitor presence is essential for accurate IL-1 quantification.
    • The findings contribute to understanding T cell activation mechanisms and IL-1's role.

    Related Experiment Videos