Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The de Broglie Wavelength02:32

The de Broglie Wavelength

33.8K
In the macroscopic world, objects that are large enough to be seen by the naked eye follow the rules of classical physics. A billiard ball moving on a table will behave like a particle; it will continue traveling in a straight line unless it collides with another ball, or it is acted on by some other force, such as friction. The ball has a well-defined position and velocity or well-defined momentum, p = mv, which is defined by mass m and velocity v at any given moment. This is the typical...
33.8K
Leaky Scanning02:28

Leaky Scanning

5.7K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.7K
Specific Heat01:16

Specific Heat

67.9K
The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
67.9K
Quantifying Heat02:46

Quantifying Heat

62.4K
Thermal Energy Microscopically, thermal energy is the kinetic energy associated with the random motion of atoms and molecules. Temperature is a quantitative measure of “hot” or “cold”, which depends on the amount of thermal energy. When the atoms and molecules in an object are moving or vibrating quickly, they have a higher average kinetic energy (KE) (or higher thermal energy), and the object is perceived as “hot”, or it is described as being at a higher temperature. When the...
62.4K
Heat Flow and Specific Heat01:12

Heat Flow and Specific Heat

6.8K
Heat is a type of energy transfer that is caused by a temperature difference, and it can change the temperature of an object. Since heat is a form of energy, its SI unit is the joule (J). Another common unit of energy often used for heat is the calorie (cal), which is defined as the energy needed to change the temperature of 1 g of water by 1 °C, specifically between 14.5 °C and 15.5 °C, since the energy needed shows a slight temperature dependence. Another commonly used unit is...
6.8K
Heating and Cooling Curves02:44

Heating and Cooling Curves

28.1K
When a substance—isolated from its environment—is subjected to heat changes, corresponding changes in temperature and phase of the substance is observed; this is graphically represented by heating and cooling curves.
For instance, the addition of heat raises the temperature of a solid; the amount of heat absorbed depends on the heat capacity of the solid (q = mcsolidΔT). According to thermochemistry, the relation between the amount of heat absorbed or released by a substance, q, and its...
28.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural changes shifting the redox potential of the outlying cluster N1a in respiratory complex I.

Structure (London, England : 1993)·2025
Same author

A leigh syndrome mutation perturbs long-range energy coupling in respiratory complex I.

Chemical science·2025
Same author

Quinone chemistry in respiratory complex I involves protonation of a conserved aspartic acid residue.

FEBS letters·2024
Same author

The Radical SAM Heme Synthase AhbD from <i>Methanosarcina barkeri</i> Contains Two Auxiliary [4Fe-4S] Clusters.

Biomolecules·2023
Same author

Exploring ND-011992, a quinazoline-type inhibitor targeting quinone reductases and quinol oxidases.

Scientific reports·2023
Same author

E. coli cytochrome bd-I requires Asp58 in the CydB subunit for catalytic activity.

FEBS letters·2022

Related Experiment Video

Updated: Feb 15, 2026

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
06:26

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

5.6K

Low cost, microcontroller based heating device for multi-wavelength differential scanning fluorimetry.

Jo Hoeser1,2, Emmanuel Gnandt3, Thorsten Friedrich4

  • 1Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, 79104, Freiburg i. Br., Germany. hoeser@bio.chemie.uni-freiburg.de.

Scientific Reports
|January 25, 2018
PubMed
Summary
This summary is machine-generated.

This study presents a low-cost, microcontroller-based heating device for differential scanning fluorimetry. This affordable setup accurately determines protein melting points across various buffer conditions using standard equipment.

More Related Videos

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

62.9K
Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
04:04

Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device

Published on: December 27, 2024

1.7K

Related Experiment Videos

Last Updated: Feb 15, 2026

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
06:26

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

5.6K
Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

62.9K
Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
04:04

Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device

Published on: December 27, 2024

1.7K

Area of Science:

  • Biochemistry
  • Protein Stability Analysis
  • Biophysical Chemistry

Background:

  • Differential scanning fluorimetry (DSF) is a common technique for assessing protein stability.
  • DSF typically requires specialized, expensive equipment like temperature-controlled fluorescence spectrometers or RT-PCR machines.
  • Estimating protein melting points is crucial for understanding protein behavior and function.

Purpose of the Study:

  • To develop and validate a low-budget, accessible method for differential scanning fluorimetry.
  • To enable protein stability assessments using readily available laboratory equipment.
  • To determine the melting points of both soluble and membranous proteins under diverse buffer conditions.

Main Methods:

  • Implementation of a microcontroller-based heating device integrated with a 96-well plate reader.
  • Connection of the modified plate reader to a standard fluorescence spectrometer.
  • Application of the system to measure protein melting points in various buffer solutions.

Main Results:

  • Successful demonstration of a low-cost alternative for differential scanning fluorimetry.
  • Accurate determination of protein melting points for both soluble and membranous protein types.
  • Validation of the method across a range of buffer conditions.

Conclusions:

  • The developed microcontroller-based heating device offers an affordable and effective approach to protein stability analysis.
  • This innovation democratizes access to differential scanning fluorimetry, making it feasible in resource-limited settings.
  • The system reliably measures protein melting points, supporting broader research in protein biophysics and drug discovery.