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Hepatitis B virus Genotypes in West Azarbayjan Province, Northwest Iran

Mohammad Hasan Khadem Ansari1, Yousef Rasmi1,2, Laya Abbasi3

  • 1Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

Open Access Macedonian Journal of Medical Sciences
|January 25, 2018
PubMed

Insights

Hepatitis B virus (HBV) genotype D is dominant in West Azarbaijan, Iran. This finding aligns with lower rates of chronic liver disease, cirrhosis, and liver cancer, aiding public health strategies.

Area of Science:

  • Hepatology
  • Virology
  • Genetics

Background:

  • Hepatitis B virus (HBV) infections pose a significant global health burden, causing millions of deaths annually from chronic hepatitis, cirrhosis, and hepatocellular carcinoma.
  • HBV transmission occurs through blood and blood products, highlighting the importance of understanding viral genotypes and mutations.
  • Genotyping of HBV is crucial for effective vaccination strategies, antiretroviral therapy, and disease prevention efforts.

Purpose of the Study:

  • To determine the prevalent genotypes and sub-genotypes of the Hepatitis B virus in patients residing in West Azarbaijan province, Iran.
  • To provide essential epidemiological data for informing public health interventions and disease management strategies.
  • To investigate the correlation between HBV genotypes and the incidence of liver-related diseases in the studied population.

Main Methods:

  • A cross-sectional study involving 100 Hepatitis B patients (70 male, 30 female) from Urmia University of Medical Sciences.
  • Serum samples were tested for Hepatitis B surface antigen (HBsAg) using ELISA.
  • DNA extraction, Sanger sequencing of S genes, phylogenetic analysis using Neighbor-joining, and Nested PCR for genotyping accuracy.

Main Results:

  • The dominant genotype identified among Hepatitis B patients in West Azarbaijan was genotype D.
  • Sequence and phylogenetic analyses confirmed the homogeneity of HBV genotypes in the Iranian population studied.
  • Results from sequencing methods were consistent with Nested PCR, ensuring reliable genotyping.

Conclusions:

  • Genotype D is the predominant Hepatitis B virus genotype in the West Azarbaijan province of Iran.
  • The prevalence of genotype D correlates with a lower incidence of acute liver disease, chronic hepatitis, cirrhosis, and hepatocellular carcinoma in the region.
  • These findings underscore the importance of genotype-specific epidemiological data for targeted public health initiatives against Hepatitis B.
Abstract

Keywords:
HBsAgHepatitis B GenotypeNested PCRSequencing

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