Related Experiment Video
Updated: Feb 15, 2026

Determination of Total Lipid and Lipid Classes in Marine Samples
Published on: December 11, 2021
LC-MS/MS Analysis of Lipid Oxidation Products in Blood and Tissue Samples
Yiu Yiu Lee1, Jetty Chung-Yung Lee2
1School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China.
Abstract:
Oxygenated lipid products of non-cyclooxygenase derivatives, namely, prostanoids such as, isoprostanes and isofurans, are formed in vivo through lipid autoxidation. Insofar it has been marked as novel biomarkers of oxidative stress in the biological systems. Elevations of these oxidized products are associated with several diseases. This chapter describes the preparation and measurement of the products, including newly identified F2-dihomo-isoprostanes and dihomo-isofurans, from plasma and tissue samples using the liquid chromatography-tandem mass spectrometry approach.
More Related Videos
17:39A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
Published on: July 16, 2017
11:17A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Related Concept Videos
Lipid Digestion
Oxidation Numbers
What are Lipids?
Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Structure of Lipids
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...