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Shotgun Lipidomics Approach for Clinical Samples.

Lars F Eggers1, Dominik Schwudke2

  • 1Division of Bioanalytical Chemistry, Research Center Borstel, Borstel, 23845, Germany.

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|January 25, 2018
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Summary

Shotgun lipidomics provides rapid and reliable lipid analysis in clinical samples. This study details a workflow optimized for tissues, plasma, and cells, enhancing biomedical research capabilities.

Keywords:
Clinical samplesHigh-resolution mass spectrometryLipid extractionLipid identificationShotgun lipidomics

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Shotgun lipidomics enables rapid and reproducible lipid identification and quantification.
  • Established methyl tert-butyl ether (MTBE) protocols are widely used for lipid extraction in shotgun lipidomics.
  • Analysis of clinical samples presents unique challenges for lipidomics workflows.

Purpose of the Study:

  • To describe a robust shotgun lipidomics workflow tailored for clinical sample analysis.
  • To optimize lipid extraction and analysis for diverse biological matrices.
  • To facilitate high-throughput lipid profiling in biomedical research.

Main Methods:

  • Development and validation of a shotgun lipidomics workflow.
  • Application of the methyl tert-butyl ether (MTBE) lipid extraction protocol.
  • Analysis of various clinical sample types including tissue, blood plasma, and peripheral blood mononuclear cells.

Main Results:

  • The described workflow demonstrates suitability for clinical sample analysis.
  • High reproducibility and speed in lipid identification and quantification were achieved.
  • The method is applicable across different sample matrices, including tissues and biofluids.

Conclusions:

  • The presented shotgun lipidomics workflow is effective for analyzing clinical samples.
  • This approach enhances the capability for lipid biomarker discovery in diseases.
  • The workflow supports reproducible and efficient lipidomic studies in clinical settings.