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Published on: March 5, 2019
MiR-361 inhibits osteosarcoma cell lines invasion and proliferation by targeting FKBP14
1Department of Surgery, Yantaishan Hospital, Yantai, China. suhao78@sohu.com.
Objective:
MicroRNAs have been reported to contribute to the development of osteosarcoma via negatively regulating the target genes. Nevertheless, the undiscovered function of miR-361 on osteosarcoma development remains uncertain.
Patients And Methods:
MiR-361 and FKBP14 (FK506-binding protein 14) expression in osteosarcoma samples were detected by Real-time polymerase chain reaction (PCR). Cells invasive ability was examined via the transwell invasion assay. The luciferase reporter assay was used to examine the regulation mechanism. The protein level of FKBP14 was detected by Western blot. Cell Counting Kit-8 (CCK-8) was used to detect cell lines proliferation.
Results:
MiR-361 was reduced both in osteosarcoma samples and cell lines. Up-regulation of miR-361 significantly inhibited cells invasive and proliferative abilities, while down-regulation of miR-361 promoted cell lines invasion and proliferation. miR-361 could negatively regulate FKBP14 in osteosarcoma. Suppression of FKBP14 could reverse the function of miR-361 inhibitor.
Conclusions:
MiR-361 inhibits osteosarcoma cell lines invasion and proliferation by targeting FKBP14.
Insights
MicroRNA-361 (miR-361) inhibits osteosarcoma progression. Lower miR-361 levels promote tumor invasion and proliferation by targeting FKBP14, a key factor in osteosarcoma development.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- MicroRNAs (miRNAs) are crucial regulators in cancer development, often acting as tumor suppressors or oncogenes.
- Osteosarcoma, a primary bone malignancy, exhibits complex molecular alterations contributing to its aggressive nature.
- The specific role of miR-361 in osteosarcoma pathogenesis remained largely unexplored, necessitating further investigation.
Purpose of the Study:
- To elucidate the function of miR-361 in osteosarcoma development.
- To identify the molecular targets and regulatory mechanisms of miR-361 in osteosarcoma cells.
- To assess the potential of miR-361 as a therapeutic target for osteosarcoma.
Main Methods:
- Quantitative Real-time PCR (qRT-PCR) was employed to measure miR-361 and FKBP14 expression levels in osteosarcoma tissues and cell lines.
- Transwell invasion assays were conducted to evaluate the impact of miR-361 on cell invasiveness.
- Luciferase reporter assays and Western blotting were utilized to confirm the direct targeting of FKBP14 by miR-361 and assess protein level changes.
- Cell Counting Kit-8 (CCK-8) assays were performed to determine the effect of miR-361 on cell proliferation.
Main Results:
- Expression of miR-361 was significantly downregulated in osteosarcoma samples and cell lines compared to normal controls.
- Overexpression of miR-361 markedly suppressed osteosarcoma cell invasion and proliferation.
- Conversely, inhibition of miR-361 enhanced cell invasion and proliferation.
- miR-361 was identified as a direct negative regulator of FKBP14 expression in osteosarcoma.
- Silencing FKBP14 partially reversed the promoting effects of miR-361 inhibition on cell invasion and proliferation.
Conclusions:
- MiR-361 functions as a tumor suppressor in osteosarcoma.
- The inhibitory effect of miR-361 on osteosarcoma progression is mediated through the downregulation of its target gene, FKBP14.
- Targeting the miR-361/FKBP14 axis presents a potential therapeutic strategy for osteosarcoma treatment.
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