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Identification of cardiac long non-coding RNA profile in human dilated cardiomyopathy

Huaping Li1, Chen Chen1, Jiahui Fan1

  • 1Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 No. Jiefang Avenue, Wuhan 430030, China.

Cardiovascular Research
|January 25, 2018
PubMed

Insights

This study identifies key long non-coding RNAs (lncRNAs) altered in dilated cardiomyopathy (DCM). These findings offer new insights into heart failure mechanisms and potential therapeutic targets for DCM.

Area of Science:

  • Cardiovascular Biology
  • Molecular Genetics
  • Genomics

Background:

  • Dilated cardiomyopathy (DCM) is a major cause of heart failure.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cardiovascular disease pathogenesis.

Purpose of the Study:

  • To compare lncRNA expression profiles in human hearts with and without DCM.
  • To investigate the functional roles of specific differentially expressed lncRNAs in cardiac cells.
  • To identify potential molecular mechanisms underlying DCM development.

Main Methods:

  • Microarray analysis of lncRNA expression in human heart samples (14 DCM, 10 control).
  • siRNA-mediated knockdown to assess lncRNA function in cardiac myocytes, fibroblasts, and endothelial cells.
  • RNA-sequencing and pathway analysis.
  • Validation of TAF10 as a regulator of lncRNA expression.

Main Results:

  • Identified 313 significantly differentially expressed lncRNAs in DCM hearts.
  • Demonstrated functional roles for lncRNAs RP11-544D21.2 and XLOC_014288 in cardiac cell function, including impaired endothelial cell tube formation and migration.
  • Confirmed TAF10 as a factor influencing lncRNA expression.
  • Found correlations between altered lncRNAs and left ventricular ejection fraction.

Conclusions:

  • Provides a comprehensive comparison of cardiac lncRNA profiles in DCM versus control.
  • Highlights the complex roles of differentially expressed lncRNAs in various cardiac cell types.
  • Suggests novel therapeutic targets and mechanistic insights for DCM treatment.
Abstract

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