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Construction of FGF21 knockout mouse models by the CRISPR/Cas9 system

Xu Liu1, Ping Zhang1, Xiao-Feng Zhang1

  • 1The Research Center for Laboratory Animal Sciense, College of Life Science, Inner Mongolia University, Hohhot 010070, China.

Yi Chuan = Hereditas
|January 26, 2018
PubMed

Insights

Fibroblast Growth Factor 21 (FGF21) plays a key role in hair follicle development. FGF21 knockout mice exhibit impaired hair growth, slower regrowth, and smaller hair follicles, highlighting FGF21

Area of Science:

  • Cell signaling and molecular biology
  • Genetics and genomics
  • Dermatology and hair biology

Background:

  • Fibroblast Growth Factors (FGFs) are crucial intercellular signaling molecules regulating physiological functions.
  • FGF21, a member of the FGF family, is implicated in hair follicle development and the hair growth cycle.

Purpose of the Study:

  • To investigate the specific roles of FGF21 in hair follicle development and the hair growth cycle.
  • To establish and characterize a FGF21 knockout mouse model for further research.

Main Methods:

  • Utilized CRISPR/Cas9 gene-editing technology to create FGF21 knockout mice.
  • Designed a targeting vector and microinjected Cas9 mRNA and gRNA into fertilized FVB mouse ova.
  • Confirmed knockout status via DNA sequencing, qRT-PCR, and Western blotting to verify null expression of FGF21.

Main Results:

  • Successfully generated three lines of Fgf21 knockout mice (Fgf21-/-) with confirmed null genotypes and phenotypes.
  • FGF21 mRNA and protein were undetectable in Fgf21-/- mouse tissues.
  • Fgf21-/- mice displayed reduced body weight, slower hair regrowth, diminished hair quantity, and smaller hair follicle diameters compared to wild-type (WT) mice.

Conclusions:

  • The established Fgf21 knockout mouse model is a valuable genetic tool for studying FGF21's functions.
  • FGF21 is essential for normal hair follicle development and regulating the hair growth cycle.
  • This model will facilitate future investigations into FGF21's precise mechanisms in hair biology.

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