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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Published on: August 28, 2018
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Construction of FGF21 knockout mouse models by the CRISPR/Cas9 system
Xu Liu1, Ping Zhang1, Xiao-Feng Zhang1
1The Research Center for Laboratory Animal Sciense, College of Life Science, Inner Mongolia University, Hohhot 010070, China.
Yi Chuan = Hereditas
|January 26, 2018
Summary
Fibroblast Growth Factor 21 (FGF21) plays a key role in hair follicle development. FGF21 knockout mice exhibit impaired hair growth, slower regrowth, and smaller hair follicles, highlighting FGF21
Area of Science:
- Cell signaling and molecular biology
- Genetics and genomics
- Dermatology and hair biology
Background:
- Fibroblast Growth Factors (FGFs) are crucial intercellular signaling molecules regulating physiological functions.
- FGF21, a member of the FGF family, is implicated in hair follicle development and the hair growth cycle.
Purpose of the Study:
- To investigate the specific roles of FGF21 in hair follicle development and the hair growth cycle.
- To establish and characterize a FGF21 knockout mouse model for further research.
Main Methods:
- Utilized CRISPR/Cas9 gene-editing technology to create FGF21 knockout mice.
- Designed a targeting vector and microinjected Cas9 mRNA and gRNA into fertilized FVB mouse ova.
- Confirmed knockout status via DNA sequencing, qRT-PCR, and Western blotting to verify null expression of FGF21.
Main Results:
- Successfully generated three lines of Fgf21 knockout mice (Fgf21-/-) with confirmed null genotypes and phenotypes.
- FGF21 mRNA and protein were undetectable in Fgf21-/- mouse tissues.
- Fgf21-/- mice displayed reduced body weight, slower hair regrowth, diminished hair quantity, and smaller hair follicle diameters compared to wild-type (WT) mice.
Conclusions:
- The established Fgf21 knockout mouse model is a valuable genetic tool for studying FGF21's functions.
- FGF21 is essential for normal hair follicle development and regulating the hair growth cycle.
- This model will facilitate future investigations into FGF21's precise mechanisms in hair biology.
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