Related Experiment Video

Updated: Feb 15, 2026

A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
08:09

A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System

Published on: May 13, 2016

7.0K

Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract

Monique Y Rennie1, Stephanie Stovall2, James P Carson3

  • 1Knight Cardiovascular Institute, Center for Developmental Health, Oregon Health & Science University, Portland, OR 97239, USA. moniquerennie@gmail.com.

Journal of Cardiovascular Development and Disease
|January 26, 2018
PubMed

Insights

Altered blood flow during embryonic development impacts heart formation. This study shows collagen changes in the embryonic heart outflow tract (OFT) adapt to hemodynamic overload, with collagens I and XIV playing key roles.

Area of Science:

  • Developmental biology
  • Cardiovascular research
  • Tissue engineering

Background:

  • Normal cardiac development relies on precise blood flow (hemodynamics).
  • Deviations from normal hemodynamic stimuli can cause congenital heart defects.
  • Early embryonic heart tissue remodeling in response to altered hemodynamics remains unclear.

Purpose of the Study:

  • To investigate collagen remodeling in the embryonic chicken heart outflow tract (OFT) under hemodynamic overload.
  • To understand the role of specific collagens (I, III, VI, XIV) in embryonic heart adaptation to altered blood flow.

Main Methods:

  • Hemodynamic overload was induced in chicken embryos (HH18-HH24) by constricting the OFT with a suture.
  • Gene and protein expression of fibril collagens (I, III) and fibril-organizing collagens (VI, XIV) were quantified using qPCR and immunofluorescence.
  • Collagen deposition and fibril organization were analyzed in relation to the degree of lumen constriction.

Main Results:

  • Collagen I levels increased upstream and in cushions of constricted OFTs.
  • Collagen VI deposition was elevated downstream of the constriction.
  • Collagen XIV expression increased throughout the OFT and correlated with constriction severity.
  • Organized collagen I fibrils were observed in regions with increased Collagen XIV in more severely constricted embryos.

Conclusions:

  • Embryonic heart tissue remodels its collagen composition in response to hemodynamic overload.
  • Collagen XIV appears crucial for adapting the embryonic heart's structure to increased pressure.
  • Collagen I and XIV may play a synergistic role in the structural adaptation of the embryonic heart to hemodynamic stress.

Keywords:
cardiac developmentcongenital heart diseaseheart malformationmechanotransductiontissue remodeling

More Related Videos

In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart06:31

In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart

Published on: April 27, 2016

7.8K
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization09:56

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Published on: September 29, 2015

9.8K

Related Concept Videos

Phase Transitions: Sublimation and Deposition02:33

Phase Transitions: Sublimation and Deposition

Some solids can transition directly into the gaseous state, bypassing the liquid state, via a process known as sublimation. At room temperature and standard pressure, a piece of dry ice (solid CO2) sublimes, appearing to gradually disappear without ever forming any liquid. Snow and ice sublimate at temperatures below the melting point of water, a slow process that may be accelerated by winds and the reduced atmospheric pressures at high altitudes. When solid iodine is warmed, the solid sublimes...
20.4K
Fibril-associated Collagen01:11

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.4K
Anatomy of the Heart01:27

Anatomy of the Heart

The human heart is made up of three layers of tissue that are surrounded by the pericardium, a membrane that protects and confines the heart. The outermost layer, closest to the pericardium, is the epicardium. The pericardial cavity separates the pericardium from the epicardium. Beneath the epicardium is the myocardium, the middle layer, and the endocardium, the innermost layer. There are four chambers of the heart: the right atrium, the right ventricle, the left atrium, and the left ventricle.
120.7K
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.7K
Nerve Supply of the GI Tract01:27

Nerve Supply of the GI Tract

The neuronal supply to the gastrointestinal (GI) tract is essential for regulating various functions, including digestion, absorption, and movement of food. This intricate network of nerves is known as the enteric nervous system (ENS), often referred to as the "second brain" of the body.
The enteric nervous system consists of two major plexuses: the myenteric plexus (Auerbach's plexus) and the submucosal plexus (Meissner's plexus). These plexuses are located within the layers of...
3.7K
Histology of the Gastrointestinal (GI) Tract01:20

Histology of the Gastrointestinal (GI) Tract

The GI tract, from beginning to end, is made up of four continuous tissue layers that adjust their structure according to their specific roles. These layers, from innermost to outermost, are known as the mucosa, submucosa, muscularis, and serosa, which are continuous with the mesentery.
The mucosa is sometimes called a mucous membrane due to its mucus-secreting features. This membrane is composed of epithelium, which directly interacts with ingested substances, and the lamina propria, a layer...
3.6K