Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vertical Curve: Problem Solving01:23

Vertical Curve: Problem Solving

527
Vertical curves provide the transition between two roadway grades, ensuring safety, comfort, and functionality. Calculating elevations at specific stations along the curve involves several systematic steps based on the curve's geometry and provided design parameters.The vertical curve is defined by its length, grades, Point of Vertical Intersection (P.V.I.) location, and P.V.I. elevation. The stations of the Point of Vertical Curvature (P.V.C.), where the curve begins, and the Point of Vertical...
527
Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.6K
Econometric Views (EViews)01:29

Econometric Views (EViews)

606
Econometric Views, often stylized as EViews, is a package that merges statistical analysis with econometric studies. It is designed to provide tools for time series analysis, forecasting, and econometric model simulation. The software originated from MicroTSP software and has evolved significantly since its inception in 1981. The history of EViews is marked by a continuous effort to enhance its computational speed and user interface. It was initially developed for large computing systems but...
606
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Introduction to Vertical Curves01:24

Introduction to Vertical Curves

628
Vertical curves are parabolic transitions that connect different grades on highways and railroads, ensuring a smooth alignment between back and forward tangents. The back tangent represents the initial grade, while the forward tangent defines the subsequent grade. These curves can be symmetrical, with equal tangent lengths, or nonsymmetrical, with varying lengths. The key points defining a vertical curve include the Point of Vertical Intersection (P.V.I.), where the tangents meet; the Point of...
628
Cranial Bones: Lateral View01:27

Cranial Bones: Lateral View

7.3K
The lateral view of the cranium is dominated by temporal, sphenoid, and ethmoid bones.
The temporal bone forms the lower lateral side of the skull. The temporal bone is subdivided into several regions. The flattened upper portion is the squamous portion of the temporal bone. Below this area and projecting anteriorly is the zygomatic process of the temporal bone, which forms the posterior portion of the zygomatic arch. Posteriorly is the mastoid portion of the temporal bone. Projecting...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular adhesion assay for biopolymer systems.

The Review of scientific instruments·2026
Same author

A survey of physical methods for studying nuclear mechanics and mechanobiology.

APL bioengineering·2021
Same author

Induction of ciliary orientation by matrix patterning and characterization of mucociliary transport.

Biophysical journal·2021
Same author

Nuclear Deformation Causes DNA Damage by Increasing Replication Stress.

Current biology : CB·2020
Same author

Combined Selective Plane Illumination Microscopy and FRAP Maps Intranuclear Diffusion of NLS-GFP.

Biophysical journal·2020
Same author

Correlating nuclear morphology and external force with combined atomic force microscopy and light sheet imaging separates roles of chromatin and lamin A/C in nuclear mechanics.

Molecular biology of the cell·2020

Related Experiment Video

Updated: Feb 15, 2026

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts
05:58

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts

Published on: March 29, 2024

1.6K

Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies.

Kellie Beicker1, E Timothy O'Brien1, Michael R Falvo1

  • 1Department of Physics and Astronomy, UNC-Chapel Hill, Chapel Hill, NC, 27599, USA.

Scientific Reports
|January 26, 2018
PubMed
Summary

This study introduces a novel system combining atomic force microscopy with advanced imaging to observe cell mechanics. It reveals correlations between applied forces and subcellular structural changes, enhancing cell mechanics models.

More Related Videos

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
07:07

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

1.9K
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
13:01

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

34.8K

Related Experiment Videos

Last Updated: Feb 15, 2026

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts
05:58

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts

Published on: March 29, 2024

1.6K
Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
07:07

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

1.9K
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
13:01

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

34.8K

Area of Science:

  • Cellular mechanics
  • Biophysics
  • Mechanobiology

Background:

  • Accurate cell mechanics models require measuring dynamic structural changes under load.
  • Standard atomic force microscopy (AFM) primarily measures vertical forces, limiting simultaneous imaging of vertical structural dynamics.
  • Existing methods struggle to correlate applied forces with subcellular structural responses in real-time.

Purpose of the Study:

  • To develop and validate a novel integrated system for simultaneous AFM force measurements and high-speed vertical plane imaging.
  • To enable synchronized observation of subcellular structural dynamics and applied forces.
  • To improve the accuracy of cell mechanics and mechanotransduction models.

Main Methods:

  • Integration of sideways imaging and vertical light sheet illumination with AFM.
  • High frame rate, low background imaging of subcellular structures in the vertical plane.
  • Synchronization of imaging data with AFM force measurements during cell compression and adhesion studies.

Main Results:

  • The system successfully captured high-speed, low-background images of subcellular dynamics synchronized with AFM force data.
  • Correlated stiffening in force-indentation data with the onset of nuclear deformation during cell compression.
  • Linked detailed force data during adhesive release to specific strain events at the cell membrane and within the nucleus.

Conclusions:

  • The developed integrated system provides unprecedented insight into cell mechanics by correlating force application with dynamic structural changes.
  • This technology advances the study of mechanotransduction by enabling detailed analysis of cellular responses to mechanical stimuli.
  • The findings pave the way for more accurate computational models of cell behavior under load.