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Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
Damayanti Chakraborty1, Masanaga Muto1, Michael J Soares1,2
1Institute for Reproductive Health and Regenerative Medicine, Departments of Pathology & Laboratory Medicine and Pediatrics, University of Kansas Medical Center, Kansas City, KS, USA.
Abstract:
In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of 'gain-of-function' and 'loss-of-function' blastocyst manipulations can be evaluated with in vitro outgrowth assays or following transfer to pseudopregnant rats.
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