Related Experiment Video

Updated: Feb 15, 2026

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
11:51

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

Published on: November 18, 2019

6.3K

Quantitative proteomics reveals that miR-222 inhibits erythroid differentiation by targeting BLVRA and CRKL

Li Jiang1, Xing Wang1, Yong Wang1

  • 1Medical Research Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Cell Biochemistry and Function
|January 26, 2018
PubMed

Insights

MicroRNA-222 (miR-222) regulates red blood cell development by targeting BLVRA and CRKL. Inhibiting miR-222 increases BLVRA and CRKL, promoting erythroid differentiation.

Area of Science:

  • Molecular Biology
  • Hematology
  • Gene Regulation

Background:

  • MicroRNA-222 (miR-222) is implicated in erythroid differentiation.
  • The specific molecular targets of miR-222 in this process are not fully understood.

Purpose of the Study:

  • To identify and validate the direct targets of miR-222 involved in erythroid differentiation.
  • To elucidate the regulatory mechanism of miR-222 in K562 cells and human CD34+ HPCs.

Main Methods:

  • Proteomics and bioinformatics analysis to identify potential miR-222 targets.
  • Transfection with miR-222 mimics and inhibitors in K562 cells and CD34+ HPCs.
  • Luciferase assays, Western blotting, and siRNA-mediated gene silencing.

Main Results:

  • BLVRA and CRKL were identified as upregulated targets of miR-222 inhibition.
  • miR-222 directly targets the 3'-UTR of BLVRA and CRKL mRNA.
  • Overexpression of BLVRA/CRKL enhanced erythroid differentiation, while silencing attenuated it.

Conclusions:

  • BLVRA and CRKL are direct targets of miR-222.
  • miR-222 regulates erythroid differentiation through the modulation of BLVRA and CRKL expression.
Keywords:
BLVRACRKLerythropoiesismiR-222microRNA

More Related Videos

A Quantitative Glycomics and Proteomics Combined Purification Strategy11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.5K
Quantitative Analysis of Chromatin Proteomes in Disease08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

13.7K

Related Concept Videos

Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.4K
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.9K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.0K
Implicit Differentiation01:25

Implicit Differentiation

In classical mechanics, motion is often described through relationships between spatial coordinates and time. A car moving along a straight highway with constant acceleration serves as a simple case where velocity is an explicit function of time. This scenario results in a linear equation, enabling straightforward analysis using basic differentiation techniques.In contrast, a satellite in circular orbit follows a path defined by an implicit function. The position of the satellite is constrained...
72
Logarithmic Differentiation01:28

Logarithmic Differentiation

When a car’s weight and driving forces act on a tire, they impose an external load on the rubber material. This load is resisted internally by forces distributed throughout the tire structure, which are defined as stress. The resulting deformation of the rubber due to this stress is quantified as strain. The relationship between stress and strain governs how the tire deforms under load and is central to understanding its mechanical response during operation.Rubber exhibits a nonlinear...
77