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Updated: Feb 15, 2026

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
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Conditioned Medium Obtained from Amnion-Derived Mesenchymal Stem Cell Culture Prevents Activation of Keloid

Chigusa Sato1,2, Yuhei Yamamoto1,2, Emi Funayama1,2

  • 1Sapporo, Japan.

Plastic and Reconstructive Surgery
|January 26, 2018
PubMed
Abstract

Insights

Amnion-derived mesenchymal stem cell conditioned medium shows potential for keloid treatment by inhibiting fibroblast proliferation and activation. This regenerative medicine approach offers a promising topical therapy for abnormal scarring.

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Dermatology

Background:

  • Mesenchymal stem cells (MSCs) are crucial in regenerative medicine.
  • MSC-conditioned medium (MSC-CM) is known to possess anti-inflammatory properties.
  • Keloids result from abnormal fibrosis driven by fibroblast activation due to inflammation.

Purpose of the Study:

  • To investigate the efficacy of amnion-derived MSC-CM in suppressing keloid fibroblast activation.
  • To assess the impact of MSC-CM on fibroblast proliferation and extracellular matrix gene expression.

Main Methods:

  • Fibroblasts from keloid, mature, and normal tissues were cultured.
  • Cells were stimulated with transforming growth factor-beta (TGF-β).
  • Effects of MSC-CM, including concentrated forms, on fibroblast proliferation, activation markers (α-smooth muscle actin), and collagen I expression were analyzed.

Main Results:

  • MSC-CM tended to suppress keloid fibroblast proliferation while up-regulating mature fibroblast proliferation.
  • MSC-CM significantly inhibited TGF-β-induced α-smooth muscle actin expression in keloid and normal fibroblasts.
  • MSC-CM suppressed TGF-β-induced collagen I expression in keloid fibroblasts.

Conclusions:

  • Amnion-derived MSC-CM effectively inhibits keloid fibroblast proliferation and activation.
  • MSC-CM demonstrates potential as a topical therapeutic agent for keloid treatment.
  • This finding supports the use of MSC-derived products in managing fibrotic disorders.

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