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Published on: April 1, 2019
MTHFR Gene Polymorphism Is Associated With DNA Hypomethylation and Genetic Damage Among Benzene-Exposed Workers in
Jing-Chao Ren1, Yu-Xia Wu, Zhenzhen Wu
1Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, School of Public Health, Xinxiang Medical University, Xinxiang (Dr Ren, Mr Wu, Dr Zhang, Ms Cui, Dr Cao); Department of Health Surveillance, The Center for Disease Control and Prevention of Putuo District (Mr Wu); Department of Occupational Health and Toxicology, School of Public Health, Fudan University (Dr Xia), Shanghai; Puyang People's Hospital, Puyang (Mr Wang, Mr Liu); Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing (Dr Chen, Dr Liu, Dr Cao), China; Department of Environmental and Occupational Health, School of Public Health, Drexel University, Philadelphia, Pennsylvania (Dr Frank).
Objective And Methods:
To analyze the association between global DNA methylation and single-nucleotide polymorphisms (SNPs) in methylenetetrahydrofolate reductase (MTHFR). MTHFR polymorphisms rs1801133 and rs1801131 were detected using the restriction fragment length polymorphism method, and cytokinesis-block micronucleus (MN) frequency and global DNA methylation was measured in workers from 410 shoe factories.
Results:
A multilinear regression analysis demonstrated that DNA methylation of the TT variant allele of rs1801133 was lower than that of the CC wild type allele (Exp(β) [95% CI], 0.76 [0.56, 1.02], P = 0.071), with a P-value approaching significance. A significantly increased MN frequency was observed for carriers of the TT genotype (frequency ratio = 1.27, 95% CI: 1.07-1.51, P < 0.01).
Conclusion:
The results imply that the TT genotype in rs1801133 is associated with global DNA hypomethylation, which may influence the induction of MN following exposure to benzene.
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