Related Experiment Video
Updated: Feb 15, 2026
05:05
A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
18.1K
[Exosomal biomarkers for kidney disease.]
Michihiro Hosojima1, Hideyuki Kabasawa1, Shoji Kuwahara2
1Department of Clinical Nutrition Science, Kidney Research Center, Niigata University, Japan.
Clinical Calcium
|January 27, 2018
Summary
Urinary extracellular vesicles (EVs) offer promising minimally invasive biomarkers for kidney disease detection and monitoring. This review highlights key urinary EV biomarkers, including megalin, for conditions like diabetic nephropathy.
Area of Science:
- Nephrology
- Biomarker Discovery
- Extracellular Vesicle Research
Background:
- Minimally invasive biomarkers are crucial for early kidney disease detection, prognosis, and management.
- Urinary extracellular vesicles (EVs), including exosomes, are increasingly studied for biomarker potential.
- Marker proteins and microRNAs (miRNAs) within urinary EVs are key research targets.
Purpose of the Study:
- To review the current landscape of urinary EV-contained biomarkers for kidney diseases.
- To highlight specific biomarkers, such as megalin, found in urinary EVs.
- To discuss the application of these biomarkers in conditions like diabetic nephropathy.
Main Methods:
- Comprehensive literature review of studies on urinary EV biomarkers.
- Analysis of marker proteins and miRNAs within urinary EVs.
- Focus on biomarkers relevant to kidney disease diagnosis and monitoring.
Main Results:
- Urinary EVs contain numerous potential biomarkers for kidney diseases.
- Proteins like megalin, a proximal tubular endocytic receptor, are significant urinary EV biomarkers.
- miRNAs within urinary EVs also show diagnostic and prognostic value.
Conclusions:
- Urinary EV biomarkers represent a significant advancement in non-invasive kidney disease assessment.
- Megalin and specific miRNAs in urinary EVs hold potential for diabetic nephropathy and other kidney diseases.
- Further research into urinary EV biomarkers will enhance early detection and personalized treatment strategies.
Related Concept Videos
01:25Chronic Kidney Disease I: Introduction
780
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
780
01:24Chronic Kidney Disease II: Clinical Manifestations
700
Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
700
01:28Chronic Kidney Disease III: Interprofessional Care
488
Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
488
01:18Chronic Kidney Disease IV: Nursing Management
416
Nursing management is essential for preventing complications, maintaining stability, and improving patients' quality of life in chronic kidney disease (CKD). By using a structured approach, nurses help slow CKD progression and support effective patient care.1. Comprehensive patient assessmentEffective management begins with nurses reviewing the patient’s medical history, and identifying key risk factors like diabetes, hypertension, and nephrotoxic drug use. Nurses assess signs of...
416
01:45Kidney Structure
75.6K
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries.
75.6K
01:36Overview of Exosomes
3.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.8K