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Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
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Cell-surface marker discovery for lung cancer.

Allison S Cohen1, Farah K Khalil2, Eric A Welsh3

  • 1Department of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.

Oncotarget
|January 27, 2018
PubMed
Summary

Researchers identified novel lung cancer cell-surface markers to develop targeted therapies. Six markers (CA9, CA12, CXorf61, GPR87, LYPD3, SLC7A11) significantly correlate with poorer patient survival, aiding personalized lung cancer treatment.

Keywords:
cell-surfacelung cancermolecular imagingtarget biomarkertargeted therapeutics

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Lung cancer remains a leading cause of cancer mortality.
  • Development of targeted therapies and molecular imaging agents is crucial for improving patient outcomes.
  • Identifying cell-surface targets is essential for effective delivery of therapeutic payloads or imaging contrast agents.

Purpose of the Study:

  • To discover and characterize novel lung cancer cell-surface markers.
  • To identify targets for developing targeted therapeutic and molecular imaging agents.
  • To enable personalized medicine approaches in lung cancer care.

Main Methods:

  • Analysis of microarray gene expression data from patient specimens to identify differentially expressed cell-surface markers.
  • Selection of ten candidate markers based on expression levels in tumors versus normal tissues and biological relevance.
  • Validation of protein expression using immunohistochemistry (IHC) on patient samples.
  • Correlation of marker expression with patient survival data using Kaplan-Meier analysis.

Main Results:

  • Over 200 putative cell-surface markers were identified as overexpressed in lung cancers.
  • Ten markers (CA9, CA12, CXorf61, DSG3, FAT2, GPR87, KISS1R, LYPD3, SLC7A11, TMPRSS4) were selected for further validation.
  • High expression of six markers (CA9, CA12, CXorf61, GPR87, LYPD3, SLC7A11) was significantly associated with worse patient survival.
  • Protein expression was confirmed in lung tumors and cell lines.

Conclusions:

  • The identified cell-surface markers represent promising targets for novel lung cancer therapeutics and imaging agents.
  • These markers can facilitate the development of personalized treatment strategies for lung cancer patients.
  • The association of six markers with poor survival highlights their potential as prognostic indicators.