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Sirt6 mRNA-incorporated endothelial microparticles (EMPs) attenuates DM patient-derived EMP-induced endothelial

Tong Jing1, Kuang Ya-Shu1, Wang Xue-Jun1

  • 1Department of Cardiology, Shanghai Tongji Hospital, Tongji University, Shanghai, China.

Oncotarget
|January 27, 2018
PubMed
Abstract

Insights

Endothelial microparticles (EMPs) from diabetic patients show low Sirt6. EMPs carrying Sirt6 mRNA protect against endothelial dysfunction by promoting angiogenesis and nitric oxide formation.

Area of Science:

  • Cardiovascular Biology
  • Metabolic Disease Research
  • Cellular Vesicle Biology

Background:

  • Endothelial microparticles (EMPs) are biomarkers for endothelial dysfunction and therapeutic targets in diabetic vascular disease.
  • Sirtuins regulate endothelial dysfunction in diabetes, but their role within EMPs is unknown.

Purpose of the Study:

  • Investigate the diagnostic value of EMPs in diabetes.
  • Determine the protective effects of Sirtuin 6 (Sirt6) mRNA-incorporated EMPs on endothelial dysfunction.

Main Methods:

  • Prepared EMPs from cultured HUVECs and blood of diabetic patients and healthy volunteers.
  • Utilized Adv-Sirt6 and Sirt6 siRNA to modulate Sirt6 expression.
  • Assessed endothelial dysfunction via angiogenesis, inflammatory markers, nitric oxide (NO) production, and eNOS phosphorylation.

Main Results:

  • Elevated EMP levels and low Sirt6 expression were observed in diabetic patients and high glucose conditions.
  • Diabetic EMPs impaired EC angiogenesis and increased inflammation.
  • Sirt6 mRNA-enriched EMPs promoted EC angiogenesis, increased eNOS phosphorylation, and reduced inflammation.

Conclusions:

  • Sirt6 mRNA-carrying EMPs show potential to ameliorate endothelial dysfunction in diabetic patients.
Keywords:
EMPsdiabetesendothelial dysfunctionsirtuin 6vascular complication

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