Subacute Inhalation Toxicity of Cyclohexanone in B6C3F1 Mice

Area of Science:

• Toxicology
• Environmental Health

Background:

• Cyclohexanone (C6H10O) is a widely used industrial chemical with limited data on repeated inhalation toxicity.
• Understanding its subacute toxicity is crucial for occupational safety and risk assessment.

Purpose of the Study:

• To characterize the subacute inhalation toxicity of cyclohexanone in B6C3F1 mice.
• To determine the no observable adverse effect level (NOAEL) following repeated exposure.

Main Methods:

• Mice were exposed to 0, 50, 150, or 250 ppm of cyclohexanone for 6 hours/day, 5 days/week, for 4 weeks (OECD Test Guideline 412).
• Evaluated parameters included mortality, clinical signs, body weight, food consumption, hematology, serum biochemistry, organ weights, and gross/histopathological findings.

Main Results:

• No mortality or significant clinical signs were observed across all exposure groups.
• No adverse effects were noted in body weight, food consumption, hematology, serum biochemistry, or gross/histopathological findings.
• Some statistically significant changes in organ weights were observed, but without corresponding adverse effects.

Conclusions:

• The no observable adverse effect level (NOAEL) for subacute cyclohexanone inhalation in mice is greater than 250 ppm.
• Cyclohexanone exhibits a low toxicity profile under the tested subacute inhalation conditions.