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Updated: Feb 15, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
Orthogonal supramolecular protein assembly on patterned bifunctional surfaces
D Wasserberg1, J Cabanas-Danés2, V Subramaniam3
1Bioinspired Molecular Engineering Laboratory, MIRA Biomedical Technology and Technical Medicine Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. p.jonkheijm@utwente.nl and Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, 7500 AE Enschede, The Netherlands and Nanobiophysics Group, MESA+ Institute for Nanotechnology and MIRA Biomedical Technology and Technical Medicine Institute, University of Twente, 7500 AE Enschede, The Netherlands.
Abstract:
We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.
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