Related Experiment Video

Updated: Feb 15, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
15:17

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

14.4K

Microarray profiles reveal that circular RNA hsa_circ_0007385 functions as an oncogene in non-small cell lung cancer

Ming-Ming Jiang1, Zhi-Tao Mai1, Shan-Zhi Wan1

  • 1Department of Respiration, Cangzhou Central Hospital, Xinhua Road, No. 201, Yunhe District, Cangzhou City, 061001, Hebei Province, China.

Journal of Cancer Research and Clinical Oncology
|January 27, 2018
PubMed
Abstract

Insights

Circular RNAs (circRNAs) are key in non-small cell lung cancer (NSCLC). Hsa_circ_0007385 promotes NSCLC growth and may be a therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are a novel class of non-protein-coding RNA involved in various pathophysiological processes.
  • Emerging evidence suggests circRNAs play regulatory roles in cancer development.

Purpose of the Study:

  • To investigate the expression profiles and pathological roles of circRNAs in non-small cell lung cancer (NSCLC).
  • To identify specific circRNAs involved in NSCLC tumorigenesis.

Main Methods:

  • circRNA microarray analysis to screen expression profiles in NSCLC tissues.
  • Quantitative reverse transcription PCR (qRTPCR) to measure circRNA and microRNA (miRNA) expression.
  • In vitro (cell proliferation, migration, invasion assays) and in vivo (xenograft) experiments to assess functional roles.
  • Bioinformatics and luciferase reporter assays to identify miRNA targets.

Main Results:

  • Microarray identified 957 differentially expressed circRNAs in NSCLC tissues.
  • Hsa_circ_0007385 was significantly upregulated in NSCLC tissues and cells.
  • Knockdown of hsa_circ_0007385 suppressed NSCLC cell proliferation, migration, and invasion.
  • In vivo studies showed hsa_circ_0007385 knockdown reduced tumor growth.
  • Hsa_circ_0007385 potentially targets miR-181.

Conclusions:

  • Hsa_circ_0007385 plays a significant role in NSCLC tumorigenesis.
  • Hsa_circ_0007385 represents a potential therapeutic target for NSCLC treatment.
Keywords:
Circular RNAHsa_circ_0007385MiR-181Non-small cell lung cancerOncogene

More Related Videos

Glycan Profiling of Plant Cell Wall Polymers using Microarrays12:30

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

15.2K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.5K
Lung Capacity01:47

Lung Capacity

The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.5K
Deformation in a Circular Shaft01:10

Deformation in a Circular Shaft

One of the distinctive characteristics of circular shafts is their ability to maintain their cross-sectional integrity under torsion. In other words, each cross-section continues to exist as a flat, unaltered entity, simply rotating like a solid, rigid slab. To understand the distribution of shearing stress within such a shaft, consider a cylindrical section inside this circular shaft. This section has a length of L and a radius of R, with one end fixed. The radius of the cylindrical section is...
948
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.3K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.3K