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Updated: Feb 15, 2026

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke
12:19

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

Published on: March 11, 2018

12.2K

Quantitative assessment of demyelination in ischemic stroke in vivo using macromolecular proton fraction mapping

Marina Y Khodanovich1, Alena A Kisel1, Andrey E Akulov1,2

  • 11 Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|January 27, 2018
PubMed

Insights

Fast macromolecular proton fraction (MPF) mapping accurately quantifies demyelination after stroke. This MRI technique shows strong correlation with histological markers, validating MPF as a key biomarker for ischemic stroke research.

Area of Science:

  • Neuroimaging
  • Stroke Research
  • Demyelination

Background:

  • Ischemic stroke causes demyelination, impacting neurological function.
  • Accurate quantification of demyelination is crucial for understanding stroke pathology and recovery.
  • Existing MRI methods require validation against histological markers.

Purpose of the Study:

  • To validate fast macromolecular proton fraction (MPF) mapping as an imaging biomarker for demyelination in a rat stroke model.
  • To compare MPF with other quantitative MRI parameters and histological markers of brain injury.
  • To assess the correlation between MPF and demyelination markers over time post-stroke.

Main Methods:

  • Utilized transient middle cerebral artery occlusion (MCAO) in rats.
  • Acquired quantitative MRI data, including MPF, T1, T2, proton density, and ADC.
  • Compared MRI parameters with histological stains (Luxol Fast Blue, Bielschowsky) and immunohistochemistry (NeuN, Iba1) at 1, 3, and 10 days post-MCAO.

Main Results:

  • MPF and Luxol Fast Blue (LFB) optical density were significantly reduced in the ischemic lesion compared to the contralateral hemisphere.
  • MPF and LFB optical density showed a strong correlation (R=0.81), validating MPF's ability to detect demyelination.
  • Incorporating T2 into regression analysis improved MPF-LFB agreement by correcting for edema.

Conclusions:

  • Fast macromolecular proton fraction (MPF) mapping is a validated imaging biomarker for demyelination in ischemic stroke.
  • MPF provides a reliable, non-invasive method for assessing demyelination severity.
  • This technique holds potential for clinical applications in stroke diagnosis and monitoring.
Keywords:
Macromolecular proton fractionhistologymagnetic resonance imagingmiddle cerebral artery occlusionmyelin

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