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Updated: Feb 15, 2026

Induction and Validation of Cellular Senescence in Primary Human Cells
Published on: June 20, 2018
Bmal1-deficient mouse fibroblast cells do not provide premature cellular senescence in vitro
Yasukazu Nakahata1, Shiori Yasukawa1, Fiqri Dizar Khaidizar1
1a Laboratory of Gene Regulation Research, Graduate School of Biological Sciences , Nara Institute of Science and Technology (NAIST) , Ikoma, Nara , Japan.
Abstract:
Bmal1 is a core circadian clock gene. Bmal1-/- mice show disruption of the clock and premature aging phenotypes with a short lifespan. However, little is known whether disruption of Bmal1 leads to premature aging at cellular level. Here, we established primary mouse embryonic fibroblast (MEF) cells derived from Bmal1-/- mice and investigated its effects on cellular senescence. Unexpectedly, Bmal1-/- primary MEFs that showed disrupted circadian oscillation underwent neither premature replicative nor stress-induced cellular senescence. Our results therefore uncover that Bmal1 is not required for in vitro cellular senescence, suggesting that circadian clock does not control in vitro cellular senescence.
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