Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.4K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
DNA as a Genetic Template02:05

DNA as a Genetic Template

28.0K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
28.0K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluticasone Propionate/Anti-IgE Combination Preserves Bone Mechanical and Mineral Integrity Better than Monotherapies or Anti-TNF-α in Mice with Ovalbumin-Induced Allergic Airway Inflammation.

International journal of molecular sciences·2026
Same author

Determination of the yearly distribution of the incidence of Allergic Bronchopulmonary Aspergillosis (ABPA) in patients registered in the cystic fibrosis patient registry system of Turkey and identification of risk factors for its development.

Chronic illness·2026
Same author

DeepAlloWeb: a web server for interactive allosteric pockets prediction using protein language model.

Journal of molecular biology·2026
Same author

The Evaluation of the Genotype and Phenotype Relationship in Patients With Nasal Polyps in Cystic Fibrosis Registry of Turkey.

Pediatric pulmonology·2026
Same author

Repurposing AI for protein interactions and dynamics: opportunities, limitations, and lessons.

Frontiers in bioinformatics·2026
Same author

Deep generative molecular design and its value in modern drug discovery.

Expert opinion on drug discovery·2026

Related Experiment Video

Updated: Feb 15, 2026

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

15.3K

PRISM-EM: template interface-based modelling of multi-protein complexes guided by cryo-electron microscopy density

Guray Kuzu1, Ozlem Keskin1, Ruth Nussinov2

  • 1Center for Computational Biology and Bioinformatics and College of Engineering, Koc University, 34450 Istanbul, Turkey.

Acta Crystallographica. Section D, Structural Biology
|January 27, 2018
PubMed
Summary

This article provides updated data for Table 6 and supplementary materials. These revisions enhance the crystallographic information presented in the original 2016 publication.

Keywords:
PRISM-EMmodelling protein assembliesmultimolecular protein complexesprotein structure predictionthree-dimensional electron microscopy

More Related Videos

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.7K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

6.0K

Related Experiment Videos

Last Updated: Feb 15, 2026

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

15.3K
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.7K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

6.0K

Area of Science:

  • Crystallography
  • Materials Science

Background:

  • The original article by Kuzu et al. (2016) presented key crystallographic data.
  • Accurate data is crucial for structural analysis and material property understanding.

Purpose of the Study:

  • To provide a revised Table 6.
  • To supply updated Supporting Information for the referenced article.

Main Methods:

  • Data revision and compilation.
  • Preparation of supplementary materials.

Main Results:

  • A revised Table 6 is now available.
  • Additional Supporting Information has been provided.

Conclusions:

  • The revisions ensure the accuracy and completeness of the crystallographic data.
  • These updates facilitate further research and application of the findings.