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Mouse chromosome fragility.

M M Sanz, E C Jenkins, W T Brown

    American Journal of Medical Genetics
    |January 1, 1986
    PubMed
    Summary
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    Researchers identified specific chromosome locations prone to damage in mouse cell lines treated with 5-fluorodeoxyuridine (FUdR). These findings reveal strain-specific fragile sites, offering a new animal model for studying chromosome fragility and genetic linkage.

    Area of Science:

    • Cytogenetics
    • Mammalian genetics
    • Molecular biology

    Background:

    • Chromosome fragility is a phenomenon where specific regions of chromosomes are prone to breakage.
    • Understanding the genetic basis and distribution of fragile sites is crucial for genetic research and disease modeling.

    Purpose of the Study:

    • To investigate strain-specific patterns of chromosome damage in mouse fibroblast cell lines.
    • To identify and characterize fragile sites induced by 5-fluorodeoxyuridine (FUdR) exposure.
    • To explore the potential of identified fragile sites as markers for genetic studies.

    Main Methods:

    • Cultures of fibroblast-like cells from inbred mouse strains (RBC/Dn and AEJ/GnRk) were treated with 5-fluorodeoxyuridine (FUdR).
    • Chromosomal analysis was performed to observe and map the distribution of gaps, breaks, and exchanges.

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  • G-banding techniques were used to identify specific chromosomal locations.

    • Main Results:

    • Non-random, strain-specific distributions of chromosome lesions (gaps, breaks, exchanges) were observed upon FUdR exposure.
    • Two novel strain-specific fragile sites were identified: one in RBC/Dn at G-band 15A2 and another in AEJ/GnRk at G-band 19B.
    • Constitutive fragile sites were found at G-bands 12A2 and 18A2 in both strains.
    • A strain-specific marker was identified at G-band 9B in the AEJ/GnRk strain.

    Conclusions:

    • The study identified specific, strain-dependent chromosomal sites susceptible to damage, indicating preferential lesion occurrence.
    • The characterized fragile sites serve as a valuable animal model for investigating the mechanisms of chromosome fragility.
    • These fragile sites and the identified marker can be utilized as polymorphic markers for future linkage studies in mice.