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Related Experiment Video

Updated: Feb 15, 2026

Author Spotlight: Studying Behavior of Acanthamoeba to Develop Targeted Strategies for Preventing Acanthamoeba Keratitis
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Optimized Protocol for Testing Multipurpose Contact Lens Solution Efficacy Against Acanthamoeba.

Daniel P Fedorko1, Jeffrey M Brocious, Katherine D Adams

  • 1Food and Drug Administration (D.P.F., J.M.B., D.L.H., and M.B.E.), Center for Devices and Radiological Health, Office of Device Evaluation, Silver Spring, MD; Food and Drug Administration (K.D.A.), Winchester Engineering Analytical Center, Winchester, MA; and Food and Drug Administration (V.M.H.), Center for Devices and Radiological Health, Office of Science and Engineering Laboratories, Silver Spring, MD.

Eye & Contact Lens
|January 27, 2018
PubMed
Summary

This study validated a reproducible protocol for testing multipurpose contact lens solution (MPS) disinfection efficacy against Acanthamoeba. The protocol effectively evaluated MPS against Acanthamoeba trophozoites, with some variability noted for cysts.

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Area of Science:

  • Ophthalmology
  • Microbiology
  • Biotechnology

Background:

  • Contact lens wear is associated with microbial keratitis.
  • Acanthamoeba is a significant pathogen causing severe visual impairment.
  • Multipurpose contact lens solutions (MPS) are crucial for disinfection.

Purpose of the Study:

  • To assess the interlaboratory and intralaboratory reproducibility of a proposed protocol for evaluating MPS disinfection efficacy against Acanthamoeba.
  • To determine the effectiveness of different MPS against Acanthamoeba trophozoites and cysts.

Main Methods:

  • Two laboratories evaluated a standardized protocol using Acanthamoeba castellanii and Acanthamoeba polyphaga.
  • Trophozoites and cysts were exposed to four MPS with varying biocidal agents for different durations.
  • Survivors were quantified using a limiting dilution method with most probable number evaluation.

Main Results:

  • The protocol demonstrated high reproducibility within and between laboratories.
  • MPS were generally more effective against Acanthamoeba trophozoites than cysts.
  • Only hydrogen peroxide-based MPS achieved a significant log kill of cysts at the manufacturer's recommended soak time (MRST).
  • Variability was observed in the efficacy against Acanthamoeba cysts across different MPS.

Conclusions:

  • The revised protocol is a well-controlled and reproducible method for assessing MPS efficacy against Acanthamoeba trophozoites.
  • The protocol shows potential for evaluating MPS disinfection, though cyst inactivation requires further investigation.