Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteases in cellular slime mold development: evidence for their involvement.

D Fong, J T Bonner

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1979
    PubMed
    Summary

    Protein degradation is vital for cellular slime mold development. Protease inhibitors disrupt differentiation, but adding amino acids often reverses these effects, suggesting proteolysis regulates development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Return to work and work productivity in patients with breast cancer: a longitudinal study (abridged secondary publication).

    Hong Kong medical journal = Xianggang yi xue za zhi·2025
    Same author

    Timely course of SARS-CoV-2 infections and vaccinations in patients with hemato-oncological diseases: analysis of a real-life cohort.

    ESMO open·2023
    Same author

    GM-CSF Promotes Immune Response and Survival in a Mouse Model of COVID-19.

    Research square·2022
    Same author

    Long-term efficacy of an education programme in improving adherence with continuous positive airway pressure treatment for obstructive sleep apnoea.

    Hong Kong medical journal = Xianggang yi xue za zhi·2018
    Same author

    Analysis of enteric disease outbreak metrics, British Columbia Centre for Disease Control, 2005-2014.

    Canada communicable disease report = Releve des maladies transmissibles au Canada·2018
    Same author

    Mathematical Modeling of Ischemia-Reperfusion Injury and Postconditioning Therapy.

    Bulletin of mathematical biology·2017

    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Biochemistry

    Background:

    • Protein degradation is crucial for cellular processes.
    • The cellular slime mold Dictyostelium discoideum is a model organism for studying differentiation.
    • Protease activity has been localized in specific regions of Dictyostelium discoideum slugs.

    Purpose of the Study:

    • To investigate the role of protein degradation in Dictyostelium discoideum differentiation.
    • To determine if protease inhibitors affect cellular slime mold development.
    • To explore the regulatory function of proteolysis in cellular slime mold differentiation.

    Main Methods:

    • Treatment of Dictyostelium discoideum with various protease inhibitors (e.g., chloroquine, tosyllysyl chloromethyl ketone, antipain, leupeptin).

    Related Experiment Videos

  • Observation of developmental stages, including sorocarp formation.
  • Addition of amino acids and glutathione to assess reversibility of inhibition.

    • Main Results:

    • Several protease inhibitors blocked normal Dictyostelium discoideum differentiation.
    • Inhibition by chloroquine and tosyllysyl chloromethyl ketone was reversed by amino acid addition.
    • Antipain and leupeptin delayed sorocarp formation, indicating a role for proteolysis.

    Conclusions:

    • Protein degradation is essential for normal differentiation in Dictyostelium discoideum.
    • Proteolysis likely plays a regulatory role in cellular slime mold development.
    • Amino acid availability influences the impact of protease inhibition on differentiation.