Related Experiment Video

Updated: Feb 15, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

12.5K

Forkhead Box F2 Suppresses Gastric Cancer through a Novel FOXF2-IRF2BPL-β-Catenin Signaling Axis

Akira Higashimori1,2, Yujuan Dong1,3, Yanquan Zhang1

  • 1Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease and Li Ka Shing Institute of Health Sciences, Shenzhen Research Institute, The Chinese University of Hong Kong, Shatin, Hong Kong.

Cancer Research
|January 28, 2018
PubMed

Insights

Forkhead box F2 (FOXF2) is silenced by DNA methylation in gastric cancer, acting as a tumor suppressor. Its methylation predicts poor survival, highlighting its role in gastric carcinogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • DNA methylation is a key mechanism in gastric cancer (GC) development.
  • Identifying epigenetically silenced genes offers insights into GC pathogenesis.

Purpose of the Study:

  • Investigate the role of forkhead box F2 (FOXF2) in gastric cancer.
  • Determine the clinical significance of FOXF2 methylation in GC patients.

Main Methods:

  • Genome-wide methylation analysis to identify methylated genes in GC.
  • In vitro studies (cell lines) and in vivo studies (xenografts) to assess FOXF2 function.
  • Analysis of Wnt signaling pathway components and E3 ligase interactions.
  • Multivariate Cox regression for prognostic analysis.

Main Results:

  • FOXF2 is frequently methylated and silenced in GC, inversely correlating with mRNA expression.
  • FOXF2 suppresses GC cell proliferation, promotes apoptosis, and inhibits tumor growth.
  • FOXF2 inhibits Wnt signaling by promoting β-catenin degradation via IRF2BPL.
  • FOXF2 hypermethylation is an independent predictor of poor survival in early-stage GC.

Conclusions:

  • FOXF2 functions as a tumor suppressor in gastric carcinogenesis.
  • FOXF2 methylation serves as a prognostic biomarker for gastric cancer patients.
  • The FOXF2-IRF2BPL-β-catenin axis is crucial for suppressing Wnt signaling in GC.

More Related Videos

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

14.5K
Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.5K

Related Concept Videos

Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
3.1K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.6K
Hypothalamic-Pituitary Axis01:37

Hypothalamic-Pituitary Axis

The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
66.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Gastric Motility01:16

Gastric Motility

Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
3.3K
Gastric Emptying01:16

Gastric Emptying

Gastric emptying occurs when the stomach gradually releases chyme into the duodenum. When the stomach is distended, it triggers the release of gastrin, a hormone that promotes gastric acid secretion to aid in digestion. Additionally, stomach distension contributes to peristaltic waves that propel gastric contents toward the pyloric region. The gastroenteric reflex, on the other hand, primarily stimulates peristalsis in the intestines, facilitating the movement of contents further along the...
4.0K