Related Experiment Video
Updated: Feb 15, 2026

Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow
Published on: June 13, 2025
Substrate-induced structural alterations of Mycobacterial mycothione reductase and critical residues involved
Arvind Kumar1, Malathy Sony Subramanian Manimekalai1, Gerhard Grüber1
1School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
Abstract:
Redox homeostasis is a prerequisite for survival of the pathogen Mycobacterium tuberculosis (Mtb) which employs the low molecular weight thiol mycothiol (MSH). The Mycobacterial NADPH-dependent mycothione reductase (MtMtr), composed of an NADPH-, FAD-, and a dimerization-domain connected by linkers, regulates the balance of oxidized-reduced MSH. Here, we demonstrate by small-angle X-ray scattering, that NADPH-binding alters the oligomeric state equilibrium of the protein with no significant overall structural change after MSH-binding. Mutation of critical residues in the linker regions of MtMtr eliminate partially or totally the NADPH-induced oligomerization effect with simultaneous effect on enzyme activity. The data provide insight into the MtMtr linker regions involved in the novel oligomerization equilibrium of the Mycobacterial enzyme.
Related Concept Videos
Residual Plots
When the residual values are plotted against the variable x, it is called a residual...
Residual Stresses
Alterations in Respiration II
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
Critical Region, Critical Values and Significance Level
In hypothesis testing, a sample statistic is converted to a test statistic using z, t, or chi-square distribution. A critical region is an area under the curve in probability distributions demarcated by the critical value. When the test statistic falls in this region, it suggests that the null hypothesis must be rejected. As this region contains all those values of the...
Altered States of Awareness
The ingestion of substances like stimulants or hallucinogens leads to chemical alterations in the brain...
Critical Values

