Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparison of some procedures detecting circulating immune complexes.

Z Swierczynska, F Milgrom

    Immunological Investigations
    |December 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Immunologic injury of renal homografts.

    The Journal of experimental medicine·2009
    Same author

    My relations with five generations of immunologists.

    Transplantation proceedings·1999
    Same author

    Humoral transplantation antibodies.

    Transplantation proceedings·1999
    Same author

    My association with Ludwik Hirszfeld, Wrocław 1945-1954.

    Archivum immunologiae et therapiae experimentalis·1998
    Same author

    Tissue suspension agglutination: a simple method to screen species-specific and organ-specific reactions.

    International archives of allergy and immunology·1997
    Same author

    "Heterophile" antigen in sera of human recipients of renal allografts.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·1996

    This study compared four tests for detecting circulating immune complexes. Combining polyethylene glycol precipitation and anti-antibody inhibition tests offers a sensitive and reliable method for identifying immune complex diseases.

    Area of Science:

    • Immunology
    • Clinical Chemistry

    Background:

    • Circulating immune complexes (CICs) are implicated in various autoimmune and infectious diseases.
    • Accurate detection of CICs is crucial for diagnosis and monitoring disease activity.

    Purpose of the Study:

    • To evaluate and compare the sensitivity and reliability of four distinct methods for detecting CICs.
    • To assess the correlation between different CIC detection assays.

    Main Methods:

    • Four tests were employed: plain polyethylene glycol (PEG) precipitation (PEGprec), immunoelectrophoresis of PEG precipitates (IEpp), anti-antibody (AA) inhibition test with sera (AA-Is), and AA inhibition test with PEG precipitates (AA-Ipp).
    • A total of 156 pathological sera (myasthenia gravis, syphilis, gastrointestinal adenocarcinomas, rheumatoid arthritis, systemic lupus erythematosus) and 51 normal sera were analyzed.

    Related Experiment Videos

  • Statistical analysis was performed to compare the results of the four tests.

    • Main Results:

    • All four tests demonstrated a high degree of correlation (p < 0.005).
    • PEGprec and IEpp showed higher sensitivity than AA-Is and AA-Ipp, attributed to their ability to detect both IgG and IgM complexes, while AA inhibition tests detect only IgG complexes.
    • When rheumatoid arthritis and SLE sera were excluded, the four tests exhibited similar sensitivity. PEGprec identified two normal sera, while other tests were negative in normal controls.

    Conclusions:

    • The simultaneous application of PEGprec, IEpp, AA-Is, and AA-Ipp provides a sensitive and reliable procedure for detecting CICs.
    • The study highlights the importance of considering immunoglobulin class specificity when interpreting CIC assay results.
    • Combined use of these assays enhances diagnostic accuracy for immune complex-related conditions.