MAPKK Inhibitor U0126 Inhibits Plasmodiophora brassicae Development
Tao Chen1, Kai Bi1, Yanli Zhao1
1State Key Laboratory of Agricultural Microbiology, and The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, PR China.
Phytopathology
|January 31, 2018
Summary
Mitogen-activated protein kinase (MAPK) signaling pathways are crucial for pathogen development. Inhibiting these pathways in Plasmodiophora brassicae significantly reduced clubroot disease severity in host plants.
Area of Science:
- Plant Pathology
- Molecular Biology
- Biochemistry
Background:
- Mitogen-activated protein kinase (MAPK) cascades regulate essential cellular processes like growth and survival.
- These pathways are well-studied in model organisms but remain uncharacterized in the plant pathogen Plasmodiophora brassicae, the causal agent of clubroot disease.
Purpose of the Study:
- To identify and characterize MAPK cascade genes in Plasmodiophora brassicae.
- To investigate the role of MAPK signaling in the pathogen's life cycle and pathogenicity.
Main Methods:
- Genome-wide identification of MAPK, MAPK kinase (MAPKK), and MAPK kinase kinase (MAPKKK) genes.
- Transcriptional profiling during different zoosporic stages.
- Yeast two-hybrid assays to determine protein interactions.
- Inhibition of MAPK signaling using U0126 and assessment of its effect on resting spore germination and clubroot severity.
Main Results:
- Seven PbMAPK, three PbMAPKK, and nine PbMAPKKK genes were identified in the P. brassicae genome.
- Several MAPK pathway genes showed preferential expression in zoosporic stages.
- A potential MAPK cascade (PbMAKKK7-PbMAKK3-PbMAK1/PbMAK3) was proposed based on interaction studies.
- Inhibition of MAPKK with U0126 suppressed resting spore germination and significantly reduced clubroot symptoms in host plants, delaying the pathogen's life cycle.
Conclusions:
- MAPK signaling pathways are essential for the growth, development, and pathogenicity of Plasmodiophora brassicae.
- Targeting MAPK pathways presents a potential strategy for managing clubroot disease.
Keywords:
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